Neurogenin2 gene-regulated Schwann cells differentiate into neurons

doi:10.3969/j.issn.2095-4344.2013.49.020

Abstract

Abstract:

BACKGROUND: It is confirmed that astrocytes can differentiate into neurons by Neurogenin2 gene regulation, suggesting that Schwann cells may also differentiate into neurons by gene regulation.
OBJECTIVE: To evaluate the feasibility of Schwann cells differentiating into neurons by Neurogenin2 gene regulation.
METHODS: Rats Schwann cells were isolated, purified and identified. Then the Schwann cells were transfected with Neurogenin2 via green fluorescent protein gene-plentivirus. To induce neuronal differentiation, the Schwann cells were cultured in serum-free Dulbecco’s modified Eagle’s medium containing epidermal growth factor, basic fibroblast growth factor and brain-derived neurotrophic factor for 2 weeks. The morphology of induced cells was observed by microscope, and myelin basic protein and neuron-specific enolase were detected by immunocytochemistry.
RESULTS AND CONCLUSION: After transfection with Neurogenin2 via green fluorescent protein gene-plentivirus and induced differentiation, immunofluorescence assay demonstrated that 12.56% of the induced cell expressed neuron-specific enolase, but the control group did not express neuron-specific enolase. Neurogenin2 gene-transfected Schwann cells can express neuron-specific enolase, suggesting Neurogenin2 gene may regulate transdifferentiation of Schwann cells into neurons.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Schwann cells, nerve regeneration, neurons, epidermal growth factor, fibroblast growth factors, cell differentiation, genes

CLC Number:

R394.2

Xu Xin-long, Xie Qing-song, Pan Hong-song, Wei Xiao-jie, Chen Zai-feng. Neurogenin2 gene-regulated Schwann cells differentiate into neurons[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(49): 8590-8595.

Figures/Tables 3

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