Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (36): 6721-6723.doi: 10.3969/j.issn.1673-8225.2010.36.016

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Acupoint transplantation of allogenic bone marrow mesenchymal stem cells: Angiogenesis and relevant cytokine changes following acute myocardial infarction

Zhang Zhe, Li Zheng, Yang Guan-lin, Chen Yan   

  1. Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Shenyang  110032, Liaoning Province, China
  • Online:2010-09-03 Published:2010-09-03
  • About author:Zhang Zhe☆, Doctor, Associate chief physician, Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China goldkey_ren@sohu. com
  • Supported by:

    the Science and Technology Research Program of Education Department of Liaoning Province, No. 2004D281

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Abstract:

BACKGROUND: The bone marrow mesenchymal stem cell (BMSC) transplantation ways mainly contain peripheral vein injection transplantation, coronary artery transplantation and intramyocardial injection transplantation, but there are limitations such as lack of target and high price.
OBJECTIVE: To observe effects of allogenic BMSC transplantation on angiogenesis and changes in related cytokines in the infarct region following rabbit acute myocardial infarction.
METHODS: The rabbit bone marrow was extracted to isolate, culture, purify, amplify, identify and label BMSCs. A total of 55 rabbits were randomly assigned to normal and operation groups. In the operation group, at 1 week following acute myocardial infarction, the rabbits were randomly divided into three groups: acupoint injection stem cell group, model control group and acupoint injection saline group. Five weeks later, myocardial tissue was obtained for immunohistochemistry BrdU staining and cardiac specific troponin T immunohistochemistry staining. Immunohistochemistry was used to detect platelet/endothelial cell adhesion molecule-1 levels in infarct myocardial tissue. Immunohistochemistry, sandwich enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction and Western blot assay were utilized to determine vascular endothelial cell growth factor. Immunohistochemistry was employed to detect basic fibroblast growth factor.
RESULTS AND CONCLUSION: BMSCs by acupoint injection could migrate towards infarct region, and differentiate into cardiac-like cells. Compared with model control group and acupoint injection saline group, platelet/endothelial cell adhesion molecule-1 expression upregulated in and surrounding the infarct tissue in the acupoint injection stem cell group. The upregulation of platelet/endothelial cell adhesion molecule-1 expression induced angiogenesis in and surrounding the infarct region. Its mechanism is to stimulate upregulation of basic fibroblast growth factor expression in myocardial tissue. Vascular endothelial cell growth factor expression did not exhibit upregulatory tendency in the infarct myocardial tissue and serum in the acupoint injection stem cell group. Thus, angiogenesis is induced by basic fibroblast growth factor and unknown factors.

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