Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (36): 6715-6719.doi: 10.3969/j.issn.1673-8225.2010.36.015

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Survival and migration of neural stem cells surrounding the lesion sites of traumatic brain injury

Zhang Li1, Zhang Hui1, Sun Li2, Luo Qiang2, Hao Xiu-qing2, Liang Feng1, Wang Xin-sheng2, Bo Ai-hua 2   

  1. 1 Department of Human Anatomy, 2 Experimental Center, Hebei North University, Zhangjiakou  075000, Hebei Province, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Zhang Hui, Master, Professor, Master’s supervisor, Department of Human Anatomy, Hebei North University, Zhangjiakou 075000, Hebei Province, China zhanghui6312@ 126.com
  • About author:Zhang Li★, Master, Assistant, Department of Human Anatomy, Hebei North University, Zhangjiakou 075000, Hebei Province, China sunli11011@yahoo. com.cn
  • Supported by:

    the Grant from Department of Education of Hebei Province, No. 2007304

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Abstract:

BACKGROUND: It is difficult to regenerate in the central nervous system of adults. Following traumatic brain injury, the number of surviving cells surrounding the lesion sites directly affects the patients’ prognosis. How to make the transplanted neural stem and progenitor cells (NSPC) survival, migration and differentiation around the cortex damage in rats models of traumatic brain injury effectively are the keys of nerve regenerated and repair research.
OBJECTIVE: To study survival, migration and differentiation of NSPC around the cortex damage in rats models of traumatic brain injury.
METHODS: Using serum-free culture technique, epidermal growth factor and basic fibroblast growth factor were added to stimulate the origin of embryonic rat forebrain NSPC growth and proliferation. Moreover, in vitro clone culture was conducted. BrdU labeling, immunohistochemistry and immunofluorescence method were used to confirm the proliferation and multi-directional differentiation potential properties of NSPC. Survival and migration of transplanted NSPC were observed around the cortex damage in rat models of Fenney’s traumatic brain injury.
RESULTS AND CONCLUSION: Neural stem and progenitor cell clones were detected by immunohistochemistry and immunofluorescence method, which showed nestin-positive and BrdU-positive reaction. They displayed NSE-positive, GFAP-positive and MAP-2-positive reaction after NSPC differentiation in vitro. BrdU-positive cells were interspersed and GFAP-positive cells number was increased surrounding the cortex damage at 7 and 14 days posttransplantation. These suggested that rat forebrain neural stem and progenitor cells are able to proliferate and differentiate into neurons and glial cells, and can survive and migrate surrounding cortex damage, showing the characteristics of integrated into brain tissue in morphological observation.

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