Abstract:

BACKGROUND: Bone marrow stromal stem cells (BMSCs) combined with acellular dermal matrix (ADM) can repair the articular cartilage defects in rabbits. Chinese medicine Duhuojisheng soup can promote cartilage repair, and improve the quality of repair.
OBJECTIVE: To further verify Duhuojisheng decoction effects on the repair of rabbit articular cartilage defects using BMSCs complex.
METHODS: Rabbit BMSCs following in vitro proliferation were compounded on ADM, which was then implanted into rabbit knee cartilage defects. Traditional Chinese medicine Duhuojisheng decoction was given, which served as a Duhuojisheng decoction + stem cell group. This study also set Duhuojisheng decoction group, composite grouped and blank group (control group). Morphological observation and histological examination were performed in repaired tissue at 4, 8, 12 weeks.
RESULTS AND CONCLUSION: After 12 weeks, the defect in the Duhuojisheng detection + stem cell group was filled with hyaline cartilage-like tissue; cartilage and subchondral bone tissue were basically repaired; repair of cartilage in histomorphology was superior to other groups. Results have indicated that BMSCs complex can repair articular cartilage defects in rabbits, and Chinese medicine Duhuojisheng decoction can promote cartilage repair, and improve the quality of repair.