Expression of fibroblast growth factor, transforming growth factor and connective tissue growth factor in lumbar ligamentum flavum hypertrophy

doi:10.3969/j.issn.2095-4344.2014.46.015

Abstract

Abstract:

BACKGROUND: Ligamentum flavum hypertrophy is one of the most important factors of lumbar spinal stenosis, but the molecular mechanism is still not very clear.

OBJECTIVE: To explore the role of basic fibroblast growth factor, connective tissue growth factor and transforming growth factor β1 in hypertrophy of the lumbar ligamentum flavum.

METHODS: The ligamentum flavum samples were divided into three groups according to different diseases: control group (acquired from the patients with lumbar spinal canal tumor, n=6), lumbar disc herniation (LDH) group (acquired from the patients with LDH, n=6) and lumbar spinal stenosis (LSS) group (acquired from the patients with LSS, n=6). Then the mRNA expressions of basic fibroblast growth factor, connective tissue growth factor, transforming growth factor β1 and collagen I, III, V of the ligamentum flavum were detected using real-time quantitative RT-PCR method. The roles of basic fibroblast growth factor, connective tissue growth factor and transforming growth factor β1 were explored.

RESULTS AND CONCLUSION: The expression of basic fibroblast growth factor mRNA in the LSS group was significantly higher than that in the LDH and control groups (both P < 0.05); the expression of connective tissue growth factor mRNA was not found statistically different among the three groups, although it was slightly higher in the LSS group (P > 0.05); the expression of transforming growth factor β1 mRNA was significantly higher in the LSS group than in the LDH and control groups (both P < 0.01). The collagen I mRNA expressed significantly higher in the LSS group than the LDH and control groups (both P < 0.05), but both the collagen III and V mRNA showed no significant difference among the three groups (P > 0.05). This study indicate that both basic fibroblast growth factor and transforming growth factor β1 play important roles in the formation process of the lumbar ligamentum flavum hypertrophy, and the main type of the collagen in the hypertrophied ligamentum flavum is collagen I.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: ligamentum flavum, spinal stenosis, transforming growth factor beta1, fibroblast growth factor 2, connective tissue growth factor

CLC Number:

R318

Jiang Yu-quan, Liu Ji-chun, Ye Xiao-jian, Hu Yue, Qu Jin-tao.

Expression of fibroblast growth factor, transforming growth factor and connective tissue growth factor in lumbar ligamentum flavum hypertrophy

[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(46): 7452-7457.

Figures/Tables 3

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