The study of proteomics in acute spinal cord injury rats by iTRAQ technology

doi:10.3969/j.issn.2095-4344.2014.49.004

Abstract

Abstract:

BACKGROUND: Isobaric tags for relative and absolute quantitation (iTRAQ) mass spectrometry technology studys the information of relevant protein according to the ion signal shows different mass-to-charge ratio in the tandem mass spectrometry analysis.

OBJECTIVE: To establish the protein spectrum of differential proteins in cerebrospinal fluid of acute spinal cord injury rat model, study the secondary injury mechanism and find an effective method of treating acute spinal cord injury from molecular level.

METHODS: Acute spinal cord injury was produced in Sprague-Dawley rats and iTRAQ technology was applied to analyze the differential proteins in cerebrospinal fluid of acute spinal cord injury rat model.

RESULTS AND CONCLUSION: Total 722 proteins have been identified in this study, including 107 differentially expressed proteins, 63 downregulated proteins and 44 upregulated proteins. There were 19 proteins related to neurogenesis, including 14 up-regulation proteins and 5 down-regulation proteins. Seven proteins contributed to the regulation of neurogenesis. The differential proteins and growth factor identified in this study can be taken as the biomarkers of acute spinal cord injury or indicators of clinical monitoring of the progression, target treatment and efficacy assessment after acute spinal cord injury.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: spinal cord injuries, cerebrospinal fluid, proteomics, isotope labeling

CLC Number:

R318

Bai Jing-jing, Wang Chong, Ding Li-wen, Song Xing-hua, Chen Jiang-tao, Zhou Yi-jun, Xu Lei-lei . The study of proteomics in acute spinal cord injury rats by iTRAQ technology [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(49): 7891-7896.

Figures/Tables 3

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