Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (38): 7096-7100.doi: 10.3969/j.issn.2095-4344.2012.38.014

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Blood compatibility of a bilirubin adsorbent column

Xu Jian-xia1, Shao An-liang1, Yin Shu-juan2, Wang Shu-qin2, Zhang Xiu-min3, Xi Ting-fei4   

  1. 1Institute for Medical Devices Control, National Institutes for Food and Drug Control, Beijing 100050, China
    2School of Information and Engineering, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China
    3Langfang Aier Blood Purification Equipment Factory, Langfang 065000, Hebei Province, China
    4Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871,China
  • Received:2012-05-03 Revised:2012-07-31 Online:2012-09-16 Published:2012-09-16
  • About author:Xu Jian-xia★, Master, Assistant researcher, Institute for Medical Devices Control, National Institutes for Food and Drug Control, Beijing 100050, China xujianxia@nicpbp.org.cn

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Abstract:

BACKGROUND: The bilirubin adsorbent column is used to reduce the high concentration of bilirubin in the blood of patients. During the healing process, the resin particle in the column has a large-scale and long-time contact with the blood of patients. Therefore, the evaluation of blood compatibility of bilirubin adsorbent column is very important.
OBJECTIVE: To assess the blood compatibility of a bilirubin adsorbent column.
METHODS: Commercially available bilirubin adsorbent column was used as product control. The resin particles in test and control bilirubin adsorbent column were put into silicified glass tubes, respectively. The empty silicified glass tubes were used as blank control. The fresh human plasma was put into silicified glass tubes in three groups and cultured on the rotary incubator in impermeable incubator at the rate of 30 r/min under 37 ℃. After 3 minutes, the plasma was taken out to detect the prothrombin time, activated partial thromboplastin time, fibrinogen and complement hemolysis CH50. The whole process was repeated three times.
RESULTS AND CONCLUSION: Compared with the blank control group, prothrombin time and activated partial thromboplastin time in the control group and experimental group were prolonged; concentration of fibrinogen and complement hemolysis CH50 in the control group and experimental group were decreased, but the difference was reducing with the increasing plasma-resin particle contact processes. Compared with control group, values of prothrombin time, activated partial thromboplastin time, fibrinogen in the experimental group were closer to those in the blank control group in three determinations. In three determinations, complement hemolysis CH50 concentration in the experimental group was larger than that in the control group at first, then insignificantly different from the control group and at last smaller than that in the control group. Results indicated that: ①The blood compatibility of the resin particles was improved with increasing plasma-resin particle contact processes. ②The resin particle in the test bilirubin adsorbent column has fewer effect on the coagulation system than the control product. In first adsorption, the influence of the test resin particle on the complement system was smaller than the control product, but the control product had a faster increase in comparison with complement in later two adsorption processes.

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