Abstract:

BACKGROUND: Staphylococcus epidermidis is the major pathogen for the infection of the material after implantation, the underlying pathogenic mechanism is to form biofilm in the material surface, single drug administration is less effective to inhibit the growth of bacteria following biofilm formation.
OBJECTIVE: To investigate the effect of fusidic sodium combining rifampicin on Staphylococcus epidermidis biofilm in vitro.
METHODS: The biofilm was cultured in vitro. Tube dilution method was used to measure the minimal inhibitory concentration of fusidic sodium and rifampicin. The biofilm at early stage and mature stage was treated by fusidic sodium, rifampicin and the combination of fusidic sodium with rifampicin, which lower than the minimal inhibitory concentration. The influence of the combined administration on biofilm at early and mature stages was investigated.
RESULTS AND CONCLUSION: Fusidic sodium and rifampicin can inhibit Staphylococcus epidermidis adhesion and biofilm formation, and have bactericidal effects on the early and mature biofilm bacteria, the combination is much better than alone (P < 0.05). The joint use of fusidic sodium and rifampicin can remarkably inhibit Staphylococcus epidermidis adhesion and biofilm formation, destroy the early and mature Staphylococcus epidermidis-biofilm and has significant bactericidal activity, it is a good choice for treatment of bacterial biofilm related infections.