Abstract:

BACKGROUND: Early used guided bone regeneration membrane composed of collagen, polylactic acid and other single components has gradually revealed its defects in the efficiency of osteogenesis, while guided bone regeneration membrane composed of collagen, polylactic acid and other absorbable materials as the carriers, in combination with hydroxyapatite, fibroblast growth factor, bone morphogenetic protein, has become a focus of the study.
OBJECTIVE: To evaluate the absorption and tissue reaction of nano-hydroxyapatite/collagen (nHAC) membrane for guided bone regeneration.
METHODS: Three kinds of guided bone regeneration membranes (nHAC, polylactic acid-polyglycolic acid, collagen) were implanted under the dorsal skin of the rats, which were respectively sacrificed on 10, 20, 30 and 45 days after implantation. Using hematoxylin-eosin staining, the specimens were assessed histologically for the quantify and quality of tissues surrounding the membranes, interface quality and degradation rate, the tissue reaction and degradation of three kinds of membranes were evaluated.
RESULTS AND CONCLUSION: There were scattered lymphocytes, but no macrophages in nHAC membrane. The surface of nHAC had some crystal calcium phosphates and the area decreased along with the time prolonging. The collagen membrane exhibited slight tissue reaction and completely degraded at 45 days. The polylactic acid-polyglycolic acid membrane infiltration increased and markedly wrapped up with tissues. The nHAC membrane has good biocompatibility and biodegradability in vitro, which meets the criterion of guided bone regeneration.