Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (42): 7829-7834.doi: 10.3969/j.issn.1673-8225.2010.42.010

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Preparation and cytotoxicity of SiO2/Nd-Fe-B core-shelled nanoparticles applied to surgical anastomosis

Xie Zhan-tao1, Li Jian-hui1, Qian Jun-min2, Huang Shi1, Wan Zhen1, Yu Liang1, Liu Chang1, Lü Yi1　   

  1. 1 Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an   710061, Shaanxi Province, China; 2 State Key Laboratory for Mechanical Behavior of Materials, School of Materials Science and Engineering, Xi'an Jiaotong University, Xi’an   710061, Shaanxi Province, China
  • Online:2010-10-15 Published:2010-10-15
  • Contact: Lü Yi, Professor, Doctoral supervisor, Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China luyi169@126.com
  • About author:Xie Zhan-tao★, Master, Physician, Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China xiezhantao@yahoo.cn
  • Supported by:

     the National Natural Science Foundation of China, No.30830099*

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Abstract:

BACKGROUND: Magnetic compression device has acted increasing attention due to its superior performance, however, its retention in vivo would adversely affect the body, and biodegradable magnetic device is expected to solve this problem.
OBJECTIVE: To product core-shelled SiO2/Nd-Fe-B nanoparticles which can be applied to surgical anastomosis and to evaluate their cytotoxicity.
METHODS: The Nd-Fe-B nanoparticles were produced with high energy ball milling method, surface modification to the Nd-Fe-B nanoparticles was performed with sol-gel method. The cytoxicity was tested using MTT method. The core-shelled SiO2/Nd-Fe-B nanoparticles were co-cultured L929 cells, to observe the distribution of SiO2/Nd-Fe-B nanoparticles in cells and pathology of subcellular organelle, as well as the metabolism of SiO2/Nd-Fe-B nanoparticles in the subculturing cells.
RESULTS AND CONCLUSION: The core-shelled SiO2/Nd-Fe-B nanoparticles were successfully produced, the result of MTT test indicated that the cytotoxicity degree of the extract of core-shelled SiO2/Nd-Fe-B nanoparticles was 1, without significant difference of absorbance value with blank control group (P > 0.05). SiO2/Nd-Fe-B nanoparticles could enter the cells though endocytosis, and distributed in the cytoplasm without nucleus, transmission electron microscopy found a slight swelling of mitochondria and expansion of endoplasmic reticulum. Subcultured cells which contained SiO2/Nd-Fe-B nanoparticles found that nanoparticles rapidly reduction during three subcultures. SiO2/Nd-Fe-B nanoparticles can be produced using high energy ball milling combined with sol-gel method. SiO2/Nd-Fe-B nanoparticles have mildly toxicity, and can meet the requirement of the national standard for medical implantation materials. SiO2/Nd-Fe-B nanoparticles could enter the cell through endocytosis, with slight influence on organelle and fast metabolism.

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