Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (3): 391-394.doi: 10.3969/j.issn.1673-8225.2011.03.003

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Growth and osteogenesis potential of bone marrow-derived mesenchymal stem cells in nano-hydroxyapatite/collagen

Mu Xiao-hong1, Zhao Zi-yi2, Xu Lin2, Wang Shuo-ren3, Zhu Ling-qun3, Chen Jiang2, Li Xiao-ping2   

  1. 1Beijing University of Chinese Medicine, Beijing   100029, China; 2Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing   100700, China; 3Central Laboratory, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing   100700, China
  • Received:2010-07-19 Revised:2010-09-14 Online:2011-01-15 Published:2011-01-15
  • Contact: Zhao Zi-yi, Doctor, Associate chief physician, Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China zhaoziyi@hotmail.com
  • About author:Mu Xiao-hong☆, Doctor, Associate chief physician, Beijing University of Chinese Medicine, Beijing 100029, China mxh__2004@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30600847

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Abstract:

BACKGROUND: Nano-hydroxyapatite/collagen (nHAC) is a kind of nano-bone material prepared according to bionic principle, its micro-structure and compositions are closely similar to natural bone, has good biocompatibility.
OBJECTIVE: To investigate the bonding of rabbit marrow-derived mesenchymal stem cells (MSCs) and nHAC cultured in vitro, and to explore the feasibility of constructing tissue-engineering bone.
METHODS: Rabbit MSCs were isolated, cultured and purified in vitro, then rabbit MSCs of third generation were seeded onto the nHAC. Their adhesion situation was analyzed by laser confocal scanning microscope and scanning electron microscope at 3, 7, 20 days.
RESULTS AND CONCLUSION: Rabbit MSCs were well combined with nHAC, cell growth could be seen under confocal scanning microscope and scanning electron microscope; nHAC can be served as a good scaffold, it can make MSCs stably grow. Rabbit MSCs grow adherently in nHAC, and show osteogenesis potential.

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