Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (3): 386-390.doi: 10.3969/j.issn.1673-8225.2011.03.002

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Comparison of pedicle fascial flap and absorbable biomembrane in constructing tissue engineering bone to repair rabbit bone defects

Meng Xian-yong, Yang Xin-ming, Qi Zhi-min, Wang Yao-yi, Bai Rui   

  1. Department of Orthopaedics, First Affiliated Hospital of Hebei North University, Zhangjiakou   075000, Hebei Province, China
  • Received:2010-07-14 Revised:2010-08-11 Online:2011-01-15 Published:2011-01-15
  • Contact: Yang Xin-ming, Professor, Master’s supervisor, Department of Orthopaedics, First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China yxm1120@sohu.com
  • About author:Meng Xian-yong★, Master, Attending physician, Department of Orthopaedics, First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China yfymxy@yahoo.cn
  • Supported by:

    Medical Scientific Research Key Program of Hebei Province Scientific Research and Development Plan by Science and Technology Bureau of Hebei Province in 2009, No. 09276102D-46*; Medical Scientific Research Key Program by Health Bureau of Hebei Province in 2010, No. 20100481*; Scientific Research and Development Guide Plan of Zhangjiakou in 2010 No.1021083D*; Scientific Research Instruction Program of Hebei North University in 2009, No. 2009037*

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Abstract:

BACKGROUND: Tissue engineering bone constructed using both pedicle fascial flap and absorbable biomembrane can effectively promote the bone defect repair, but there is not enough study about the comparison between them.
OBJECTIVE: To observe the bone reparative effect of tissue engineering bone wrapped by pedicle fascial flap and by simple biomembrane.
METHODS: A 1-cm bone defect model was produced in rabbit bilateral ulna middle segment and periosteum. Autologous red bone marrow was implanted in the osteoinductive absorbing material containing bone morphogenetic protein to prepare uncellular tissue engineering bone, which was then implanted into the bilateral bone defect areas. The left side was wrapped by pedicle fascial flap as the experiment group, while the right side was wrapped by simple absorbable biomembrane as the control group. At 4, 8, 12, 16 weeks after the operation, each group was examined by X-ray radiograph, gross morphology and histological examination, bone shape measurement analysis in the reparative area was performed, the biomechanical detection was done at 12 weeks to compare the repair of bond defect in two groups.
RESULTS AND CONCLUSION: The experiment group was superior to the control group regarding the X-ray radiograph, gross morphology and histological inspection, the quantity and the forming speed of bone trabecula and cartilaginous tissues, the formation of mature bone structure, the remodeling of diaphysis structure, the recanalization of cavum ossis, the absorption and degradation of the implant, the ratio of new bone trabecula area to total reparative area and the final biomechanical strength (P < 0.05). The outcomes indicated that the technology of membrane guided bone regeneration using pedical fascial flap to repair large-segment bone defect can restrict the growth of fibrous connective tissue in the reparative process, and promote the effective and fast vascularization, accordingly accelerate the process and the effect of transforming implanted bone to autologous bone.

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