Effect of carbamide peroxide and hydrogen peroxide bleaching agents on laser-induced fluorescence in dentin Raman spectroscopy

doi:10.12307/2026.512

Abstract

Abstract: BACKGROUND: Hydrogen peroxide-based bleach is widely used in clinical tooth whitening treatment, but its mechanism of action has not been uniformly determined so far.
OBJECTIVE: To examine the effects of two bleaching agents, neutral 10% carbamide peroxide and neutral 40% hydrogen peroxide, on teeth and explore the mechanism of teeth whitening.
METHODS: Forty-five discarded teeth extracted for orthodontic treatment were collected, and the dentin sections were made and polished. After removing the smear layer, they were randomly divided into three groups. The control group (n=15) was placed in deionized water; the carbamide peroxide group (n=15) was placed in a neutral 10% carbamide peroxide solution, and the hydrogen peroxide group (n=15) was placed in a neutral 40% hydrogen peroxide solution. The treatment was continued for 6 hours every day for 1 week. The Raman absolute intensity and Raman relative intensity of the three groups of samples were detected by Raman spectrometer every day, and the fluorescence background intensity (the difference between the Raman absolute intensity and the Raman relative intensity) was calculated. The change trend of the amide peak of the three groups of samples every day was recorded by infrared spectrometer.
RESULTS AND CONCLUSION: (1) The relative Raman intensity of the three groups did not change significantly during the 1-7 days of treatment. The fluorescence background intensity of the control group did not change significantly during the 1-7 days of treatment. The fluorescence background intensity of the carbamide peroxide group showed a downward trend during the 1-7 days of treatment. The fluorescence background intensity of the hydrogen peroxide group decreased significantly during the 1-4 days of treatment and did not change significantly thereafter. (2) The peak areas of amide I and amide III did not change significantly during the 1-7 days of treatment in the control group. The peak areas of amide I and amide III showed a downward trend during the 1-7 days of treatment in the carbamide peroxide group and the hydrogen peroxide group, and the downward trend was more obvious in the hydrogen peroxide group. (3) The results show that the change in the laser-induced fluorescence background intensity of dentin Raman spectroscopy may be derived from the non-collagen components in dentin, and under the action of whitening agents, it may have a certain adverse effect on tooth tissue.

Key words: tooth bleaching, hydrogen peroxide, carbamide peroxide, dentin, Raman, infrared spectroscopy, engineered dental material

CLC Number:

Li Ruiqiang, Yin Chen, Ma Yan. Effect of carbamide peroxide and hydrogen peroxide bleaching agents on laser-induced fluorescence in dentin Raman spectroscopy[J]. Chinese Journal of Tissue Engineering Research, 2026, 30(2): 296-302.

Figures/Tables 4

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