Influenza A virus recombinant hemagglutinin 1 induces the production of beta-defensin and interferon-gamma in mouse tracheal epithelial cells

doi:10.12307/2025.922

Abstract

Abstract: BACKGROUND: β-defensin has the ability against influenza A virus and inhibits a series of inflammatory responses induced by influenza A virus infection within cells. There have been no reports on whether hemagglutinin 1 from influenza A virus can induce the secretion of mouse β-defensin and interferon-γ when acting in mouse tracheal epithelial cells.
OBJECTIVE: To investigate the effect of recombinant hemagglutinin 1 on the production levels of mouse β-defensin and interferon-γ in mouse tracheal epithelial cells.
METHODS: Primary mouse tracheal epithelial cell were divided into six groups: blank control group (Control), recombinant hemagglutinin 1 group (200 ng/mL), recombinant hemagglutinin 1 + influenza A virus group, influenza A virus group (2×TCID50), recombinant hemagglutinin 1 + inactivated influenza A virus group, and inactivated influenza A virus (I) group. After the mouse tracheal epithelial cells in each group were treated for 4, 8, or 24 hours, hematoxylin-eosin staining was used for pathological observation. The mRNA levels of mouse β-defensin 2, 3, and 4, and interferon-γ were detected by qRT-PCR. The protein levels of mouse β-defensin 2, 3, and 4 were measured by enzyme-linked immunosorbent assay, and the protein expression of interferon-γ was detected by western blot.
RESULTS AND CONCLUSION: (1) The recombinant hemagglutinin 1 acting alone or in combination with influenza A virus could cause different pathological changes in tracheal epithelial cells. Phenomena such as vacuolation, nuclear pyknosis and cell fusion could be observed in the cells. (2) Compared with the control group, recombinant hemagglutinin 1 alone or in combination with influenza A virus or inactivated influenza A virus significantly induced the production of mouse β-defensin 2, 3, and 4 (P < 0.05) and interferon-γ in mouse tracheal epithelial cells (P < 0.05). These results indicate that recombinant hemagglutinin 1 alone or in combination with influenza A virus can induce the production of mouse β-defensin 2, 3, and 4 and interferon-γ in mouse tracheal epithelial cells.

Key words: influenza A virus, recombinant hemagglutinin 1, β-defensin, interferon-γ, tracheal epithelial cells, mouse

CLC Number:

Lei Qi, Zhao Bingbing, Luo Hong, Chen Qiang, Jiang Yan. Influenza A virus recombinant hemagglutinin 1 induces the production of beta-defensin and interferon-gamma in mouse tracheal epithelial cells[J]. Chinese Journal of Tissue Engineering Research, 2025, 29(32): 6905-6912.

Figures/Tables 4

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