Chinese Journal of Tissue Engineering Research ›› 2025, Vol. 29 ›› Issue (31): 6601-6608.doi: 10.12307/2025.673

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Calcined deer antler slices promote proliferation of bone marrow mesenchymal stem cells

Shao Xuekun1, Shi Dianhua2, 3, Ding Zhiping1, Qiu Zhuoya1, Wang Ping2, Wang Yi2, Wang Cheng2, Ding Xiaoyan2, Sun Tiefeng1, 2   

  1. 1Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong Province, China; 2Shandong Institute of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China; 3The key Unit for Research of Technique and Principle of Honey-processing and Carbonizing of SATCM, Jinan 250014, Shandong Province, China
  • Received:2024-07-02 Accepted:2024-08-13 Online:2025-11-08 Published:2025-02-17
  • Contact: Wang Ping, MD, Researcher, Shandong Institute of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China; Co-corresponding author: Shi Dianhua, MD, Researcher, Shandong Institute of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China; The key Unit for Research of Technique and Principle of Honey-processing and Carbonizing of SATCM, Jinan 250014, Shandong Province, China
  • About author:Shao Xuekun, MS, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong Province, China
  • Supported by:
    Shandong Provincial Key R&D Program (Science and Technology Demonstration Project), No. 2021SFGC1205 (to WP); Jinan City “New Colleges and Universities 20” Funding Project Introduction Innovation Team Plan, No. 202333006 (to CP); Jinan City “New Colleges and Universities 20” Funding Project, No. 202228121 (to WP); Taishan Scholars Project Funding, No. tstp20231239 (to CP) 

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Abstract: BACKGROUND: Through scientific research addressing the effect of calcined deer antler slices on promoting the proliferation of bone marrow mesenchymal stem cells, it aims to provide empirical support for the integration and innovation of traditional Chinese medicine and modern regenerative medicine, and promote the widespread application of traditional Chinese medicine in the treatment of skeletal system diseases.
OBJECTIVE: To investigate the effect of calcined deer antler slices on bone marrow mesenchymal stem cell proliferation. 
METHODS: Different calcination samples were prepared by wrapping deer antler slices with materials such as clay, yellow clay, and salted yellow clay, resulting in seven different samples (clay-cotton cloth, yellow clay-cotton cloth, salted yellow clay-cotton cloth, yellow clay-tin foil, salted yellow clay-tin foil, yellow clay-honey roasted, salted yellow clay-honey roasted antler slices). Water-soluble extract content in deer antler slices was determined before and after calcination. CCK-8 assay was used to evaluate the effects of different aqueous extracts of calcined antler slices on the proliferation activity of bone marrow mesenchymal stem cells.
RESULTS AND CONCLUSION: (1) Calcination significantly increased the water-soluble extract content of deer antler slices, with the highest content observed in samples treated with yellow clay and honey. (2) Calcined deer antler slices significantly promoted bone marrow mesenchymal stem cell proliferation, among which the yellow clay-honey roasted deer antler slices have the most significant effect on promoting the proliferation of bone marrow mesenchymal stem cells.

Key words: bone marrow mesenchymal stem cell, cell proliferation, antler slice, calcined, yellow clay, honey roasting, water extract, engineered stem cell

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