Chinese Journal of Tissue Engineering Research ›› 2025, Vol. 29 ›› Issue (4): 730-737.doi: 10.12307/2025.257

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Biocompatibility of poly(vinylidene fluoride) piezoelectric bionic periosteum prepared by electrospinning

Zhao Shuai1, Li Dongyao2, Wei Suiyan3, Cao Yijing4, Xu Yan5, Xu Guoqiang6, 7   

  1. 1Department of Stomatology, Qingdao Jimo District People’s Hospital, Qingdao 266200, Shandong Province, China; 2Urumqi Stomatology Hospital, Urumqi 830000, Xinjiang Uygur Autonomous Region, China; 3Department of Oral Radiology, 6Department of Prosthodontics and Implantology, First Affiliated Hospital (Affiliated Stomatological Hospital) of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; 4Dental Hospital Affiliated to Nanchang University (Department of Mucosal Periodontitis), Nanchang 330000, Jiangxi Province, China; 5College of Mechanical Engineering, Xinjiang University, Urumqi 830047, Xinjiang Uygur Autonomous Region, China; 7Insitute of Stomatology, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2023-12-06 Accepted:2024-02-19 Online:2025-02-08 Published:2024-05-30
  • Contact: Xu Guoqiang, Chief physician, Associate professor, Department of Prosthodontics and Implantology, First Affiliated Hospital (Affiliated Stomatological Hospital) of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Insitute of Stomatology, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Zhao Shuai, Master, Physician, Department of Stomatology, Qingdao Jimo District People’s Hospital, Qingdao 266200, Shandong Province, China
  • Supported by:
    National Natural Science Foundation of China, No. 51965057 (to XY)

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Abstract: BACKGROUND: Our previous studies have found that poly(vinylidene fluoride) bionic periosteum prepared by electrospinning has good cytocompatibility, but its biocompatibility is unknown.
OBJECTIVE: To evaluate the biocompatibility of poly(vinylidene fluoride) bionic periosteum doped with Zn2+ and Mg2+. 
METHODS: Poly(vinylidene fluoride), poly(vinylidene fluoride) bionic periosteum doped with 1% Zn2+, doped with 1% Mg2+, and doped with 1% (Zn2++Mg2+) were prepared by electrospinning to make bionic periosteum extract. SD rats were selected as the experimental subjects for hemolysis test, short-term systemic toxicity test, and heat source test. Guinea pigs were selected as the experimental subjects for skin sensitization test. The biocompatibility of bionic periosteum of four groups was tested. 
RESULTS AND CONCLUSION: (1) The hemolysis test results showed that the hemolysis rates of 1% Zn2+ poly(vinylidene fluoride), 1% Mg2+ poly (vinylidene fluoride), 1% Zn2++1% Mg2+ poly(vinylidene fluoride) bionic periosteum and poly(vinylidene fluoride) extract were (0.130±0.013)%, (0.149±0.020)%, (0.466±0.018)%, and (0.037±0.018)%, respectively, which met the hemocompatibility standard of biomaterials. (2) The results of short-term systemic toxicity test showed that the four groups of bionic periosteal extract had no toxic signs such as body mass reduction, food intake changes, and dyspnea in SD rats, and had no toxic effects on major organs of rats. (3) Heat source test results showed that after intervention with poly(vinylidene fluoride) bionic periosteum doped with 1% Zn2+, doped with 1% Mg2+, and doped with 1% (Zn2++Mg2+),  and poly(vinylidene fluoride) bionic periosteum extract, the elevated body temperature values of SD rats were (0.133±0.058), (0.100±0.010), (0.300±0.010), and (0.300±0.017) °C, respectively. All were less than 0.6 °C, and the total temperature increase was less than 1.4 °C. (4) The results of skin sensitization test showed that no erythema or edema was observed under the skin of guinea pigs after the intervention of bionic periosteum extract of four groups. (5) The results showed that poly(vinylidene fluoride) and poly(vinylidene fluoride) bionic periosteum doped with Zn2+ and Mg2+ had good biocompatibility. 

Key words: electrospinning, poly(vinylidene fluoride), bionic periosteum, zinc ion, magnesium ion, biocompatibility 

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