Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (50): 9325-9328.

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Proliferative activity of normal human osteoblasts cultured with neuropeptides at different concentrations

Zhang Xue-min1, Ma Wen-hui2, Shi Shu-shan3, Li Ya-fei3   

  1. 1 Hebei National Defense Construction Hospital, Shijiazhuang  050081, Hebei Province, China; 2 Department of Orthopedics, Bethune International Peace Hospital, Shijiazhuang  050082, Hebei Province, China; 3 Department of Orthopedics, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing  100072, China
  • Online:2010-12-10 Published:2010-12-10
  • About author:Zhang Xue-min, Associate chief physician, Hebei National Defense Construction Hospital, Shijiazhuang 050081, Hebei Province, China hmq318@hotmail. com

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Abstract:

BACKGROUND: A large number of studies have demonstrated that neural factors can regulate bone metabolism. To date, 5 types of neuropeptides have been found to participate in bone metabolism.
OBJECTIVE: To verify the effects of neuropeptides on the biological activities of normal osteoblasts in vitro.
METHODS: The osteoblasts were stimulated by calcitonin gene-related peptide, substance P, vasoactive intestinal peptide, neusopeptide Y, noradrenaline, and bone morphogenetic protein (BMP) with concentrations of 0.02, 0.1, 1 mg/L, respectively. The effects of different factors single or associated with BMP were examined by the means of methyl thiazolyl tetrazolium.
RESULTS AND CONCLUSION: The five neuropeptides and BMP could promote osteoblast proliferation. The effect of neusopeptide Y was the strongest, and the effect of other factors was negatively related with dose. Results revealed that neuropeptides and BMP could augment the activities of osteoblasts at the different extent. This could help to explain the osteoblastic improvement of neuropeptides.

CLC Number: 

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R318