Abstract:

BACKGROUND: Uterine ischemia-reperfusion injury can lead to energy metabolism dysfunction, formation of a large number of free radicals and apoptosis, etc.
OBJECTIVE: To establish a simple, functional and reliable rat model of uterine ischemia-reperfusion injury.
METHODS: Fifty healthy Wistar rats were randomly divided into five groups. The uterine ischemia group (three groups): uterus artery of the models in the uterine ischemia group 1 was ligatured for 45 minutes by suture method after laparotomy, celiac artery of the models in uterine ischemia group 2 was ligatured for 30 minutes and the celiac artery of the models in uterine ischemia group 3 was ligatured for 45 minutes. In uterine ischemia-reperfusion group, the celiac artery was ligatured for 45 minutes by suture method after laparotomy and then reperfusion for 60 minutes. In sham-operation group, the uterine blood supply artery was not blocked.
RESULTS AND CONCLUSION: The uterine artery slim was due to the tight ligation, uterine artery rupture was due to the loose ligation which could not fully block the blood, so these two methods were not suitable to establish a uterine ischemia-reperfusion injury model. Hematoxylin-eosin staining showed that uterine cells and stroma was swelling, and neatly arranged muscle fibers could be seen after the celiac artery was ligatured for 30 minutes; in uterine ischemia-reperfusion group and celiac artery ligation for 45 minutes group, significant edema could be seen in the uterine cells and stroma cells, the exudation was increased and the muscle fiber was arranged in disorder, a massive neutrophil infiltration could be seen. Spectrophotometer detection showed that the content of uterine malondialdehyde was significantly increased in uterine ischemia-reperfusion group and celiac artery ligation for 45 minutes group (P < 0.01). It indicated that the rat uterine ischemia-reperfusion injury model could be established through reperfusion after the celiac artery was ligatured for 30 minutes by suture method.