Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (33): 6158-6163.doi: 10.3969/j.issn.2095-4344.2012.33.016

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Effect of nuclear factor E2-related factor 2 on skin carcinoma induced by long-term midwave ultraviolet irradiation

Xu Xue-zhu1, Tang Yun1, Jiang Li-li1, Li Xue-li1, Yang Hong-mei1, Kawachi Yasuhiro2○, Otsuka Fjio2○   

  1. 1Department of Dermatology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China;
    2Department of Dermatology, Department of Clinical Medicine of the University of Tsukuba, Tsukuba 305-8575, Japan
  • Received:2012-04-20 Revised:2012-06-16 Online:2012-08-12 Published:2012-08-12
  • About author:Xu Xue-zhu☆, Doctor, Associate professor, Chief physician, Master’s supervisor, Department of Dermatology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China xuxuezhu@hotmail.com

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Abstract:

BACKGROUND: Ultraviolet irradiation is an important environmental factor causing skin tumor, which is associated with the induction of oxidatie stress reaction. Nuclear factor E2-related factor 2 (Nrf2) is a major transcription factor to adjust cellular antioxidant responses and Kelch-like ECH-associated protein 1 is the specific receptor, and the relation of Nrf2-Kelch-like ECH-associated protein 1 antioxidant system and skin ultraviolet damage is close recently.
OBJECTIVE: To observe the DNA oxidative damage and photocarcinogenesis by ultraviolet irradiation on the skin of the mice, and to research the effect of Nrf2 transcription factor on skin carcinoma induced by long-term midwave ultraviolet irradiation.
METHODS: Female 8-week-ofd Nrf2 gene-deficient (Nrf2-/-) BALB/c mice and age-matched female wild-type (Nrf2+/+) BALB/c mice were selected, then the midwave ultraviolet irradiation of 100 mJ/cm2 was given to the back of mice for 4 hours; ultraviolet irradiation of 300 mJ/cm2 was given to the back of mice three times a week for 36 weeks
RESULTS AND CONCLUSION: The number of 8-hydroxy-2’-deoxyguanosine positive in Nrf2-/- mice was significantly higher than that in Nrf2+/+ mice (P < 0.05). There was no significant difference between Nrf2-/- and Nrf2+/+ mice in the mean number of tumors per animal and the incidence rate of tumors (P > 0.05). The histopathology of skin tumors was similar in two groups. This result indicates that Nrf2 has antioxidant protective effect on acute ultraviolet irradiation-induced DNA oxidative damage, there may be various factors regulating the activity of Nrf2 transcription factor in chronic ultraviolet irradiation carcinogenic process, and it needs many further studies.

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