Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (33): 6116-6120.doi: 10.3969/j.issn.2095-4344.2012.33.008

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Ginsenoside Rg1 promotes the proliferation of cultured adult human articular chondrocytes in vitro

Su Xiao-chuan1, 2, Wang Yi-sheng1, Guo Yan-xing3, Cao Xiang-yang3   

  1. 1Department of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China;
    2Department of Traditional Chinese Medicine, the Fourth Affiliated Hospital of Zhengzhou University, Zhengzhou 450044, Henan Province, China;
    3Luoyang Orthopedic-Traumatological Hospital, Luoyang 471002, Henan Province, China
  • Received:2012-04-19 Revised:2012-07-06 Online:2012-08-12 Published:2012-08-12
  • Contact: Wang Yi-sheng, Professor, Chief physician, Doctoral supervisor, Department of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China wangyisheng@zzu.edu.cn
  • About author:Su Xiao-chuan☆, Studying for doctorate, Department of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China; Department of Traditional Chinese Medicine, the Fourth Affiliated Hospital of Zhengzhou University, Zhengzhou 450044, Henan Province, China 786484578@qq.com

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Abstract:

BACKGROUND: The main active ingredient of ginseng saponins is ginsenoside Rg1 that has the advantages of promoting cell proliferation, anti-aging, anti-apoptotic, anti-inflammatory and anti-free radical. However, the previous experimental objects are often the articular chondrocytes of mice, rabbits and other animals.
OBJECTIVE: To observe the effect of ginsenoside Rg1 on the proliferation of in vitro cultured adult articular chondrocytes.
METHODS: The adult articular chondrocytes were cultured in vitro and divided into two groups: experimental group and control group. The experimental group was cultured with DMEM medium containing 40 mg/L ginsenoside Rg1, and the control group was cultured only with DMEM culture medium. After enzyme digestion, blood count plate was used to count cells, and cell growth curve plotted daily to the 7th day was observed by inverted phase contrast microscope. Type Ⅱ collagen expression in chondrocytes was detected on the 6th day.
RESULTS AND CONCLUSION: After 2 days of incubation, cells in the control group entered the logarithmic growth phase, and on the 7 th day, the cells number was about eight times that on the inoculation day. The cell proliferation capacity of the experimental group enhanced after 2 days, which was higher than that of the control group (P < 0.05), and on the 7 th day, the number of cells was about 18 times that on the inoculation day (P < 0.05). Immunohistochemical SABC results showed that both groups had type Ⅱ collagen expression in the cytoplasm, and there was no significant difference between the two groups (P > 0.05). Ginsenoside Rg1 could promote the proliferation of articular chondrocytes.

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