Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (33): 6111-6115.doi: 10.3969/j.issn.2095-4344.2012.33.007

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Effects of polydatin on the biological features of cultured fibroblasts

Bian Hui-ning, Sun Chuan-wei, Chen Hua-de, Zheng Shao-yi, Liu Zu-an, Lai Wen   

  1. Department of Burns, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China
  • Received:2012-03-14 Revised:2012-06-10 Online:2012-08-12 Published:2012-08-12
  • Contact: Lai Wen, Doctor, Associate chief physician, Department of Burns, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China gdburns@163.com
  • About author:Bian Hui-ning☆, Doctor, Associate chief physician, Department of Burns, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China bianhuining@21cn.com

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Abstract:

BACKGROUND: Polydatin has been widely used for treatment of burns and trauma. For its complicated composition, it is difficult to further study the specific pharmacological effects of polydatin. There is no study about local treatment of wound healing with polydatin.
OBJECTIVE: To investigate the effects of polydatin with different concentrations on the biological features of cultured fibroblasts.
METHODS: Fibroblasts were obtained by the explants culture of human skin and subcultured from four patients treated with scar excision skin grafting after burns. The second passage fibroblasts were cultured in culture media with varied doses of polydatin (10-6, 10-5, 10-4, 10-3, 10-2 mol/L respectively). Cultured media without polydatin was as control. The proliferation of cultured fibroblasts in vitro was detected by MTT test. The cellular cycle and the apoptosis percent were measured by flow cytometry. ELISA method was used to detect the expression of secreted collagen Ⅰ, collagen Ⅲ and fibronectin in supernatant.
RESULTS AND CONCLUSION: Polydatin with the concentration of 10-5 mol/L and 10-4 mol/L could enhance fibroblast proliferation in vitro. The absorbance value in 10-2 mol/L polydatin group was decreased and the cell growth was inhibited. Polydatin with the concentration of 10-3 mol/L could block the cellular cycle in S phase. Polydatin with the concentration of 10-2 mol/L could induce cells to apoptosis. The expressions of collagen Ⅰ, collagen Ⅲ were significantly increased in 10-2 mol/L polydatin group when compared with other groups (P < 0.05); the expression of fibronectin was significantly increased in 10-2 mol/L polydatin group when compared with control group (P < 0.05), and when compared with other polydatin groups, the expression of fibronectin was decreased (P < 0.05). Low concentration of polydation could promote fibroblast proliferation and protect the cells from apoptosis, and it could also improve the expression, synthesis and secretion of fibronectin. To fibroblasts, the optimal concentration of polydatin is 10-5 mol/L and 10-4 mol/L

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