Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (44): 6667-6672.doi: 10.3969/j.issn.2095-4344.2016.44.019

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Relationship of cell membrane microparticles CD31 and CD54 with alcohol-induced avascular necrosis of the femoral head: study protocol for a randomized controlled trial

Yang Yun1, Fan Hai-yan2, Huang Jian1, Ma Zhong-ping1, Zhang Zhi-feng1   

  1. 1Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; 2Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • Revised:2016-08-16 Online:2016-10-28 Published:2016-10-28
  • Contact: Huang Jian, M.D., Chief physician, Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • About author:Yang Yun, Master, Attending physician, Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China Fan Hai-yan, Master, Attending physician, Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China Yang Yun and Fan Hai-yan contributed equally to this paper.
  • Supported by:

    the Medical and Health Research Project of the Inner Mongolia Autonomous Region Health and Family Planning Commission, No. 201301061; the Youth Innovation Fund Project of Inner Mongolia Medical University, No. YKD2015QNCX028

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Abstract:

BACKGROUND: Cell membrane microparticles CD31 and CD54 lead to microvascular injury in the femoral head by mediating vascular inflammatory response, promoting blood clotting, affecting vasomotion and promoting vascular endothelial injury. Studies have verified that membrane particles play an important role in steroid-induced necrosis of the femoral head, but there is no studies concerning relationship between microparticles and alcohol-induced avascular necrosis of femoral head.

METHODS/DESIGN: This is a randomized controlled animal study. Healthy male Wistar rats will be randomly assigned to two groups. In the model group, rats will be intragastrically administered hard liquor for 6 consecutive months to prepare models of alcohol-induced avascular necrosis of the femoral head. Blank controls will be intragastrically given an equal volume of physiological saline. In 1-6 months of intervention, six rats will be randomly selected from each group every month. Blood will be collected separately. Flow cytometry will be used to detect serum cell membrane particles CD31, CD54 levels. Bilateral femoral head will be fixed, decalcified, embedded in wax, and then sections. After hematoxylin-eosin staining, empty bone lacuna will be quantified under a light microscope to identify femoral head necrosis. Verhoff’s staining and MSB microthrombosis staining will be used to observe microvascular injury and microvascular thrombosis in the femoral head, and to analyze the correlation of CD31 and CD54 levels with femoral head necrosis, vascular endothelial injury and microvascular thrombosis.
DISCUSSION: This study will investigate the effects of CD31 and CD54 on alcohol-induced avascular necrosis of the femoral head, explore the pathogenesis of alcohol-induced avascular necrosis of the femoral head, provide a new theoretical basis for early diagnosis and early treatment, and may provide a new target for its treatment.
ETHICS APPROVAL: The protocol has been approved by the Animal Experimental Ethics Committee of Inner Mongolia Medical University (approval number YKD2016154). Experimental procedures and materials of rats will be in accordance with the Guide for the Care and Use of Laboratory Animals, which is consistent with the guide of National Institutes of Health. 

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

Key words: Femur Head Necrosis, Membrane Proteins, Tissue Engineering

CLC Number: