Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (16): 2598-5602.doi: 10.3969/j.issn.2095-4344.2015.16.026

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Toxicity of blastula culture medium to mouse embryos: a safety evaluation of in vitro assisted reproduction devices

Han Qian-qian1, Yin Yan-yun2, Wang Han1, Feng Xiao-ming1, Wang Chun-ren1, Yang Zhao-peng1   

  1. 1Department of Biomaterials and Tissue Engineering, Institute of Medical Device Verification, the National Institutes for Food and Drug Control, Beijing 100050, China; 2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China
  • Received:2015-01-29 Online:2015-04-16 Published:2015-04-16
  • About author:Han Qian-qian, M.D., Associate researcher, Department of Biomaterials and Tissue Engineering, Institute of Medical Device Verification, the National Institutes for Food and Drug Control, Beijing 100050, China

Abstract:

BACKGROUND: The blastula culture medium can assist the development of zygote from the fertilized egg to the blast blastula. The safety and quality of blastula culture medium directly influences the quality of blastula.
OBJECTIVE: To evaluate the effect of blastula culture medium on the development of mouse embryos.
METHODS: In this study, B6D2F1 mice were used. The female mice were superovulated and mated with male B6D2F1 mice. One day later, the zygotes were collected and cultured in the M16 medium to 4-cell stage. Then, 4-cell stage embryos were transferred into the tested blastula culture medium (experimental group). After 5 days of culture, the forming rate of blastula was examined. Meanwhile, the M16 medium containing endotoxin was used to culture 1-cell mouse zygote (positive control group). The M16 medium with no embryo toxicity was used to culture 1-cell zygote (negative control group).
RESULTS AND CONCLUSION: The formation rate of blastula was 0 in the positive group, 87.1% in the negative control group, and 87.3% in the experimental group. From the results, the tested blastula culture medium could assist the 1-cell zygote growing to the stage of blastula, and the formation rate of blastula was above 80%. The tested blastula culture medium had no toxicity to the mouse embryo.


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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Key words: Blastula, Reproduction, Reproductive Techniques, Assisted

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