Inhibitory effects of sinomenine hydrochloride in T-cell acute lymphoblastic leukemia CEM cells and transcriptomic analysis

doi:10.12307/2025.661

Abstract

Abstract: BACKGROUND: Sinomenine hydrochloride has anti-tumor effects, but it is rarely reported in T-cell acute lymphoblastic leukemia.
OBJECTIVE: To investigate the inhibitory effect of sinomenine hydrochloride on CEM cells in acute T lymphoblastic leukemia.
METHODS: Different concentrations (0.5, 1, 2 and 4 mmol/L) of sinomenine hydrochloride were used to act on CEM cells. CCK-8 assay was used to detect the inhibition rate of cell proliferation and calculate the IC50. Inverted microscope and Giemsa staining were used to observe the changes of CEM cells. The RNA sequencing was performed to analyze the differential gene expression and biological information. Combined with transcriptome sequencing analysis results, flow cytometry was used to detect the apoptosis rate of CEM cells after treatment with of different concentrations (1, 2, and 4 mmol/L) of sinomenine hydrochloride. Western blot assay was used to detect the expression of Bcl-2, Bax, and Caspase-9 proteins in CEM cells after treatment with of different concentrations (1, 2, and 4 mmol/L) of sinomenine hydrochloride.
RESULTS AND CONCLUSION: (1) Sinomenine hydrochloride inhibited the growth of CEM cells in dose and time-dependent manner. (2) After dosing, the number of CEM cells decreased and pyknosis appeared. (3) RNA sequencing revealed 53 differential expressed genes. Gene Ontology was significantly enriched in cellular process, cellular anatomical entities, and binding. Signaling pathway analysis related to tumor was apoptosis. (4) Sinomenine hydrochloride induced the apoptosis of CEM cell in a concentration-dependent manner. (5) Sinomenine hydrochloride could promote the expressions of Bax and Caspase-9, but restrain the expression of Bcl-2 in CEM cells. Therefore, sinomenine hydrochloride can induce apoptosis in CEM cells and suppress cell proliferation, maybe via up-regulation of the protein levels of Bax and caspase-9 and down-regulation of the protein level of Bcl-2.

Key words: sinomenine hydrochloride, T-cell acute lymphoblastic leukemia, CEM cell, apoptosis, RNA sequencing, proliferation inhibition, engineered cell

CLC Number:

Kang Linzhi, Liu Zhenshuai, Wei Jiaxu, Chang Na, Zhu Dacheng. Inhibitory effects of sinomenine hydrochloride in T-cell acute lymphoblastic leukemia CEM cells and transcriptomic analysis[J]. Chinese Journal of Tissue Engineering Research, 2025, 29(31): 6674-6680.

Figures/Tables 7

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