Chinese Journal of Tissue Engineering Research ›› 2025, Vol. 29 ›› Issue (11): 2301-2309.doi: 10.12307/2025.353
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Ping Xingfeng, Lyu Junying, Li kai, Huang Zongxuan, Yin Jianxin
Received:
2024-03-09
Accepted:
2024-05-11
Online:
2025-04-18
Published:
2024-08-12
Contact:
Lyu Junying, Chief physician, Professor, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
About author:
Ping Xingfeng, Master, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
Supported by:
CLC Number:
Ping Xingfeng, Lyu Junying, Li kai, Huang Zongxuan, Yin Jianxin. Establishment and evaluation of a rat model of phlegm-heat and Fu-organ excess syndrome following ischemic stroke[J]. Chinese Journal of Tissue Engineering Research, 2025, 29(11): 2301-2309.
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2.1 实验动物数量分析 整个实验过程中正常对照组、痰热腑实证组无大鼠脱失,缺血性脑卒中组死亡2只、不符合纳入条件4只,病证结合组死亡6只,最终纳入结果分析48只大鼠。 2.2 各组大鼠一般指标观察及比较 空白对照组大鼠一般状态良好、被毛鲜亮有光泽,活动灵敏,呼吸平顺,鼻子未见明显分泌物,未闻及喉中痰鸣。缺血性脑卒中组大鼠被毛暗淡杂乱无光泽,呼吸急促,精神萎靡,行动迟缓,活动明显减少,鼻周未见明显分泌物,未闻及喉中痰鸣。病证结合组大鼠被毛变黄杂乱无光泽,呼吸急促,精神萎靡,烦躁,活动减少,行动迟缓,鼻周可见大量分泌物,可闻及厚重的喉中痰鸣。痰热腑实证组大鼠被毛变黄无光泽,呼吸稍急促,精神萎靡,烦躁,活动减少,行动迟缓,鼻周分泌物增多,可闻及喉中痰鸣。 2.3 各组大鼠饮食量与饮水量比较 与空白对照组比较,病证结合组和痰热腑实证组大鼠日均饮食量、日均饮水量均显著减少(P < 0.01);与缺血性脑卒中组比较,病证结合组和痰热腑实证组大鼠日均饮食量、日均饮水量均显著减少(P < 0.01),见图1。 2.4 各组大鼠体质量与体温比较 见图2。 与空白对照组比较,病证结合组、痰热腑实证组大鼠第7,14,21,28天的体质量均增加(P < 0.01),缺血性脑卒中组无明显变化(P > 0.05)。与缺血性脑卒中组比较,病证结合组、痰热腑实证组大鼠第7,14,21,28天的体质量增加(P < 0.01)。病证结合组与痰热腑实证组大鼠体质量比较差异无显著性意义(P > 0.05)。 与空白对照组比较,缺血性脑卒中组大鼠第7,14,21,28天的体温均无明显变化(P > 0.05)。与缺血性脑卒中比较,病证结合组大鼠第21,28天的体温升高(P < 0.01),痰热腑实证组大鼠第28天的体温升高(P < 0.05)。 2.5 各组大鼠粪便粒数、Bristol评分及含水率比较 与空白对照组比较,病证结合组和痰热腑实证组大鼠的粪便粒数、Bristol评分及含水率均降低(P < 0.01),缺血性脑卒中组无明显变化(P > 0.05);与缺血性脑卒中组比较,病证结合组和痰热腑实证组大鼠的粪便粒数、Bristol评分及含水率均降低(P < 0.01);病证结合组与痰热腑实证组大鼠的粪便粒数、Bristol评分及粪便含水率比较差异无显著性意义(P > 0.05),见图3。 2.6 各组大鼠舌像分析比较 与空白对照组比较,缺血性脑卒中组、病证结合组和痰热腑实证组大鼠的舌象R、G、B值均降低(P < 0.01),缺血性脑卒中组和病证结合组大鼠的舌下脉络评分升高(P < 0.01);与缺血性脑卒中组比较,痰热腑实证组大鼠的舌下脉络评分降低(P < 0.05);与病证结合组比较,痰热腑实证组大鼠的舌下脉络评分降低(P < 0.05),见图4。 2.7 各组大鼠血脂水平比较 与空白对照组比较,缺血性脑卒中组大鼠血清总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇水平比较差异均无显著性意义(P > 0.05),病证结合组和痰热腑实证组大鼠血清总胆固醇、三酰甘油、低密度脂蛋白胆固醇水平升高(P < 0.05);与缺血性脑卒中组比较,病证结合组和痰热腑实证组大鼠血清总胆固醇、三酰甘油水平升高(P < 0.05),痰热腑实证组大鼠低密度脂蛋白胆固醇水平升高(P < 0.05),见图5。 2.8 各组大鼠神经功能缺损评分比较 与空白对照组比较,缺血性脑卒中组和病证结合组大鼠造模后2,24 h的神经功能缺损评分均升高(P < 0.01),痰热腑实证组无明显变化(P > 0.05);与缺血性脑卒中组比较,病证结合组大鼠造模后2,24 h的神经功能缺损评分无明显变化(P > 0.05),见图6。 2.9 各组大鼠脑组织病理改变 苏木精-伊红染色结果显示,空白对照组、痰热腑实证组大鼠脑组织皮质区结构清晰,形态完整,细胞核大而圆,未见细胞变性、炎性浸润及间质水肿等;缺血性脑卒中组、病证结合组大鼠脑组织皮质区组织结构疏松,细胞大片坏死,细胞核碎裂或溶解,细胞质固缩、空洞化,可见明显的炎性浸润和间质水肿,见图7。 2.10 各组大鼠颈动脉组织病理改变 空白对照组大鼠颈动脉结构完整未见明显变化,内膜表面光滑平整,中层平滑肌细胞排列整齐,呈长型或椭圆型,细胞质红染,细胞核蓝染,未见明显脂质沉积;缺血性脑卒中组大鼠颈动脉血管内膜稍有增生,但不明显,可见少量炎性浸润,中层平滑肌细胞向内侧迁移,外膜结缔组织未见明显改变;病证结合组大鼠颈动脉内膜增厚严重,中层平滑肌细胞排列紊乱向内膜大量迁移增生,可见少量泡沫细胞;痰热腑实证组大鼠颈动脉内膜可见明显增生增厚,中层平滑肌细胞排列紊乱,大量增生,中膜变薄,可见少量泡沫细胞侵入,内壁可见不稳定斑块形成,见图8。 2.11 各组大鼠脑组织TTC染色及梗死率比较 TTC染色结果显示,空白对照组、痰热腑实证组染色均匀,未见明显梗死灶,缺血性脑卒中组及病证结合组均可见大面积白色梗死区域,梗死区域主要位于大脑皮质区;与空白对照组比较,缺血性脑卒中组、病证结合组梗死率升高(P < 0.01),痰热腑实证组无梗死率无明显变化(P > 0.05),见图9。 2.12 各组大鼠血清胃动素与生长抑素水平比较 与空白对照组比较,缺血性脑卒中组血清胃动素、生长抑素水平无明显变化(P > 0.05),病证结合组和痰热腑实证组血清胃动素水平下降(P < 0.01)、生长抑素水平升高(P < 0.05);与缺血性脑卒中组比较,病证结合组和痰热腑实证组血清胃动素水平下降(P < 0.01),血清生长抑素水平升高(P < 0.01),见图10。"
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