Abstract: BACKGROUND: With the widespread use of platelet transfusion, patients with ineffective platelet transfusion are increasing year by year.
OBJECTIVE: To provide a reference for improving the effect of platelet transfusion in patients with ineffective platelet transfusion by detecting and analyzing platelet antibody and cross-matching.
METHODS: The platelet antibody test kit (solid phase agglutination method) was used to detect platelet antibody and cross-matching test in 112 patients with ineffective platelet transfusion from April to December 2020.
RESULTS AND CONCLUSION: (1) The results of platelet antibody test were positive in 103 of 112 cases. The positive rate was as high as 92%. (2) The results of the platelet cross-matching type showed that 25 patients were incompatible with the donor’s platelets in the cross-matching experiment, and 18 patients were matched with all the donor’s platelets. (3) Relationship between platelet antibody test results and matching rate: The results of platelet antibody test were 84% in patients with negative platelet antibody test, 75.4%, 71.6, 54.6%, and 27.6% in patients with 1+, 2+, 3+, and 4+ platelet antibody test. Differences between groups were statistically significant (P < 0.05). (4) Relationship between the positive rate of platelet antibodies and the patients’ multiple examinations: The positive rate of platelet antibody was 90.2% and 94.2% in the number of platelet transfusions < twice and ≥ twice (P > 0.05). (5) Effect of platelet infusion on the patient’s matching rate: In each group with the same number of inspections, there was no statistically significant difference in the matching rate between the initial inspection and repeated inspections (P > 0.05). In all patients with repeated inspections, the average matching rates of the initial inspection and repeated inspections were 41.2% and 50.6%, respectively, and the difference was not statistically significant (P > 0.05). (6) These results suggest that the higher the positive degree of platelet antibody test, the lower the matching rate was. The positive intensity of platelet antibody did not show an increasing trend after infusion of matched platelets. The patient obtained a stable transfusion effect. Accurate detection of platelet antibody and infusion of matched platelets have an important clinical value for improving the efficacy of platelet transfusion.

Key words: platelet transfusion, ineffective platelet transfusion, platelet antibodies, platelet cross-matching