Allogeneic bone marrow mesenchymal stem cell transplantation can improve the function of the aging heart

doi:10.3969/j.issn.2095-4344.2016.06.008

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells can secrete a variety of factors in the local lesion, and these factors can promote cell proliferation and inhibit cell apoptosis.
OBJECTIVE: To observe the curative effect of bone marrow mesenchymal stem cell transplantation on the aging heart of rats and to explore the possible mechanism of action.
METHODS: Thirty Sprague-Dawley rats were randomized into three groups: normal blank group, model group and treatment group. Aging models were made in the latter two groups by injection of D-galactose. Rats in the treatment group were given allogeneic bone marrow mesenchymal stem cell injection, once a week, totally four times. At 1 week after final injection, the heart tissues were sliced into sections to observe the pathological changes using hematoxylin-eosin staining. Western blot assay was used to detect the expression of basic fibroblast growth factor in the heart tissues. Real-time PCR was used to measure the expression of p53 mRNA in the heart tissues.
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cell transplantation could improve the pathological morphology of the aging heart. Compared with the model group, the expression of basic fibroblast growth factor in the heart tissues was significantly higher in the treatment group (P < 0.05), but the mRNA expression of p53 was lower (P < 0.05). It is speculated that bone marrow mesenchymal stem cells can interact with heart cells to secrete basic fibroblast growth factor and reduce p53 mRNA expression, thereby playing a curative effect on the aging heart.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Li Yan-ju, Ding Yuan-ting, Zhou Yuan, Wang Fei-qing, Zeng Qiang-wu, An Shi-gang, Liu Yang . Allogeneic bone marrow mesenchymal stem cell transplantation can improve the function of the aging heart[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(6): 814-819.

Figures/Tables 4

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