Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (5): 743-747.doi: 10.3969/j.issn.2095-4344.2016.05.023

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Effects of astragalus injection on human immortalized cervical epithelial cell apoptosis in vitro 

Lv Ling1, Xiao Chen-guang2, Liu Qing1, Zhang Li3, Li Neng-lian3, She Ya-li3   

  1. 1Maternal and Child Health of Gansu Province, Lanzhou 730050, Gansu Province, China; 2Lanzhou Uranium Worker Hospital of China National Nuclear Corporation, Lanzhou 730065, Gansu Province, China; 3Traditional Chinese Medical College of Gansu, Lanzhou 730000, Gansu Province, China
  • Received:2015-11-27 Online:2016-01-29 Published:2016-01-29
  • Contact: Lv Ling, Master, Chief physician, Maternal and Child Health of Gansu Province, Lanzhou 730050, Gansu Province, China
  • About author:Lv Ling, Master, Chief physician, Maternal and Child Health of Gansu Province, Lanzhou 730050, Gansu Province, China
  • Supported by:

     the Traditional Chinese Medicine Administration Project of Gansu Province, No. GZK-2013-56

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Abstract:

BACKGROUND: Immortalized cervical epithelial cells H8 can become cancerous under the induction of carcinogenic agent, and may cause cervical cancer when there is a cofactor interaction. However, there is still a lack of effective intervention for female patients with precancerous lesions, and this treatment is blank in the clinic. 
OBJECTIVE: To explore the effects and mechanism of astragalus injection on apoptosis of human immortalized cervical epithelial cells H8.
METHODS: This study contained two groups: astragalus drug group and the blank control group. (1) Enzyme linked immunosorbent assay (ELISA) was used to detect DNA fragments of apoptotic H8 after astragalus injection. (2) Enzyme-labeling instrument was used to analyze the changes in caspase-3 and caspase-9 activities a fter astragalus injection. (3) Western blot assay was used to detect the protein expression changes of caspase-3, caspase-9 and PARP in H8 cells after astragalus injection. 
RESULTS AND CONCLUSION: (1) ELISA results showed that at 0, 6, 12 and 24 hours after 20 g/L astragalus injection, DNA fragments were gradually increased with time prolonged in a time-dependent effect (P < 0.05). (2) Enzyme-labeling instrument demonstrated that at 0, 6, 12 and 24 hours after 20 g/L astragalus injection, caspase-3 and caspase-9 activities increased in a time-dependent manner (P < 0.05). (3) At 0, 6, 12 and 24 hours after 20 g/L astragalus injection, the expression of cleaved caspase-3 and cleaved caspase-9 were gradually increased in H8 cells (P < 0.05). Cleaved PARP protein expression was gradually decreased (P < 0.05). These findings indicate that astragalus injection could obviously induce H8 apoptosis, which may be associated with the upregulated protein expression of caspase-3 and caspase-9.