In vitro destructive effect of ultrasound on Staphylococcus aureus biofilms

doi:10.3969/j.issn.2095-4344.2016.03.011

Abstract

Abstract:

BACKGROUND: Studies have shown that the ultrasound enhances the on bactericidal activity of antibiotics in an intensity-dependent manner, that is, the higher the ultrasound intensity, the greater its effectiveness.

OBJECTIVE: To study the destructive effect of different intensities of ultrasound on Staphylococcus aureus and its biofilm.

METHODS: Staphylococcus aureus biofilms were cultured in vitro using guide sheet culture method and divided into three groups for intervention. The biofilm in the control group received no treatment. The biofilm in the low-intensity ultrasound group was intervened by pulsed ultrasound with an intensity of 500 mW/cm and frequency of 200 kHz for 10 minutes. The biofilm in the high-intensity ultrasound group was intervened by continuous ultalsound with an intensity of 40 W and frequency of 1 MHz for 10 minutes. Bacterial colonies were counted using ultrasonic oscillation-live bacteria counting method. DNA and polysaccharide of the bacteria were respectively marked using propidium iodide and FITC-ConA. The molding of the bilfilm was determined using laser scanning confocal microscope.

RESULTS AND CONCLUSION: The number of bacterial colonies in the high-intensity ultrasound group were lower than that in the control and low-intensity ultrasound groups (P < 0.05), and there were no significant differences between control and low-intensity ultrasound groups. There were no significant differences in the number and intensity of red fluorescence and green fluorescence between low-intensity ultrasound and control groups; however, the number and intensity of red fluorescence and green fluorescence in the high-intensity ultrasound group were significantly decreased compared with the low-intensity ultrasound and control groups. These results demonstrate that the low-intensity ultrasound cannot kill the bacteria and it has a tiny destructive effect on the biofilm of bacteria; however, the high-intensity ultrasound can effectively kill the bacteria and has a strong destructive effect on the bilfilm of bacteria.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Zhang Wei, Wei Peng, Chen Lu . In vitro destructive effect of ultrasound on Staphylococcus aureus biofilms[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(3): 364-369.

Figures/Tables 1

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