Novel lithium chloride/calcium phosphate cement: physicochemical and osteogenic properties

doi:10.3969/j.issn.2095-4344.2016.03.001

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (3): 307-313.doi: 10.3969/j.issn.2095-4344.2016.03.001

Novel lithium chloride/calcium phosphate cement: physicochemical and osteogenic properties

Li Li, Li Bai-chuan, Wang Ren-chong, Li Bing

the Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work’s Hospital, Liuzhou 545005, Guangxi Zhuang Autonomous Region, China

Received:2015-11-16 Online:2016-01-15 Published:2016-01-15
Contact: Li Bing, M.D., Professor, Chief physician, Master’s supervisor, the Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work’s Hospital, Liuzhou 545005, Guangxi Zhuang Autonomous Region, China
About author:Li Li, Studying for master’s degree, the Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work’s Hospital, Liuzhou 545005, Guangxi Zhuang Autonomous Region, China Li Bai-chuan, Master, Chief physician, the Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work’s Hospital, Liuzhou 545005, Guangxi Zhuang Autonomous Region, China Li Li and Li Bai-chuan contributed equally to this work.
Supported by:
the National Natural Science Foundation of China, No. 81260273; the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2013GXNSFAA019269

Abstract

Abstract:

BACKGROUND: Lithium chloride is a widely used inorganic ion inhibitor of glycogen synthase kinase-3β, and it can be combined with glycogen synthase kinase-3β to activate the classical Wnt/β-catenin pathway, thereby promoting human bone marrow mesenchymal stem cells and osteoblasts proliferation and accelerating bone repair.
OBJECTIVE: To observe the physicochemical properties of novel lithium chloride/calcium phosphate cement, and to explore its osteoinductive biological property.
METHODS: Calcium phosphate cement served as control group, and lithium chloride/calcium phosphate cement containing different lithium content as experimental groups. The setting time and compressive strength of bone cement in each group were detected, and the microstructure of the material surface observed under scanning electron microscopy. Bone cement and MC3T3-E1 cells were co-cultured in vitro, and the growth and adhesion morphology of MC3T3-E1 cells on the surface of bone cement were observed under the scanning electron microscope. Effect of bone cement extracts on cell proliferation was determined through MTT assay, and alkaline phosphatase kit used for determining alkaline phosphatase activity.
RESULTS AND CONCLUSION: Lithium chloride/calcium phosphate cement had the same physicochemical properties to the calcium phosphate cement. Initial and final setting time, compressive strength and morphology of bone cement had no significant differences among groups. MC3T3-E1 cells grew and adhered well on the material surface. Results of MTT assay showed that compared with the calcium phosphate cement, the lithium chloride/calcium phosphate cement was better to improve osteoblast proliferation in vitro. In addition, the alkaline phosphatase activity in MC3T3-E1 cells was higher in experimental groups than the control group. These findings indicate that lithium chloride/calcium phosphate cement can maintain good physicochemical properties, and release lithium ions to promote bone formation.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Li Li, Li Bai-chuan, Wang Ren-chong, Li Bing. Novel lithium chloride/calcium phosphate cement: physicochemical and osteogenic properties [J]. Chinese Journal of Tissue Engineering Research, 2016, 20(3): 307-313.

Figures/Tables 5

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Novel lithium chloride/calcium phosphate cement: physicochemical and osteogenic properties

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