Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (2): 208-212.doi: 10.3969/j.issn.2095-4344.2016.02.010

Previous Articles     Next Articles

Expression of myeloperoxidase, soluble intercellular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid during maxillary canine distal movement

Xu Huan-xi1, Xing Hong-bo2, Miao Fang3, Li Ning1, Qiu Jing-yi4, Li Juan4   

  1. 1Department of Stomatology, Shihua General Hospital of Lanzhou, Lanzhou 730060, Gansu Province, China; 2Department of Stomatology, Weihai Municipal Hospital, Weihai 264200, Shandong Province, China; 3Department of Special Consultation, Lanzhou Stomatology Hospital, Lanzhou 730000, Gansu Province, China; 4State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2015-11-12 Online:2016-01-08 Published:2016-01-08
  • Contact: Li Juan, M.D., Associate professor, State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Xu Huan-xi, Attending physician, Department of Stomatology, Shihua General Hospital of Lanzhou, Lanzhou 730060, Gansu Province, China
  • Supported by:

     the Scientific Support Project of Sichuan Province, No. 2014SZ0019; the National Natural Science Foundation of China, No. 31370992

PDF

477

Abstract:

BACKGROUND: To dynamically monitor the varying levels of inflammatory factors in the gingival crevicular fluid is helpful to assess the early effect of orthodontic tooth movement. Myeloperoxidase, soluble intercellular adhesion molecule-1, pentraxin 3 are proven to be closely related to inflammation, but it is unclear about the levels of these three kinds of inflammatory factors as well as association of these three kinds of inflammatory factors with orthodontic tooth.
OBJECTIVE: To detect the expression levels of myeloperoxidase, soluble intercellular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid during maxillary canine distal movement and to assess their correlation with periodontal disease, canine movement distance and orthodontic force.
METHODS: Twenty-one orthodontic patients were enrolled and assigned into 150 g (n=12) or 100 g (n=9) groups according to orthodontic force. The gingival crevicular fluid samples of orthodontic patients were collected before and at 4, 12, 24 hours, 7, 14 days after maxillary canine distal movement. Levels of myeloperoxidase, soluble intercellular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid were measured and analyzed using ELISA assay.
RESULTS AND CONCLUSION: During the distal movement of maxillary canine, under orthodontic force, the level of myeloperoxidase was peaked at 4 hours and then decreased, while the expression level of soluble intercellular adhesion molecule-1 was peaked at 12 hours, and then decreased. Both myeloperoxidase and soluble intercellular adhesion molecule-1 levels returned to normal at 7 days under orthodontic force. The expression level of pentraxin-3 was increased significantly under orthodontic force, peaked at 24 hours, and then decreased gradually to the normal level at 7 days. In addition, the expression levels of myeloperoxidase, soluble intercellular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid were significantly higher under 150 g force than under 100 g force. These findings indicate that detecting varying levels of myeloperoxidase, soluble intercellular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid is useful to assess the efficiency of orthodontic treatment and prevent adverse reactions.