Expression of jumonji domain-containing histone demethylase 2 and estrogen-related receptor alpha in postmenopausal osteoporosis

doi:10.3969/j.issn.2095-4344.2016.02.003

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (2): 167-172.doi: 10.3969/j.issn.2095-4344.2016.02.003

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Expression of jumonji domain-containing histone demethylase 2 and estrogen-related receptor alpha in postmenopausal osteoporosis

Tang Hong-yu^{1, 2}, Dong Lu-jue³, Huo Shao-chuan³, Guo Cheng³, Zhou Chi^{1, 2}, Chen Jian-fa¹, Liu Yong³, Wang Hai-bin^{1, 2}

¹Orthopedics Center, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; ²the National Key Research Laboratory of Orthopedics, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; ³Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China

Received:2015-10-16 Online:2016-01-08 Published:2016-01-08
Contact: Wang Hai-bin, M.D., Chief physician, Professor, Doctoral supervisor, Orthopedics Center, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; the National Key Research Laboratory of Orthopedics, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
About author:Tang Hong-yu, Master, Physician, Orthopedics Center, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; the National Key Research Laboratory of Orthopedics, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
Supported by:
the National Natural Science Foundation of China, No. 81373655; Guangdong Provincial Science and Technology Plan Projects, No. 2013B021800226

Abstract

Abstract:

BACKGROUND: Jumonji domain-containing histone demethylase (JMJD) can promote osteoblast differentiation, and estrogen-related receptor alpha (ERRα) can promote osteoblast differentiation and increase bone formation. However, little is reported on the association between postmenopausal osteoporosis and JMJD and ERRα.

OBJECTIVE: To study the changes in the JMJD2 family expression in patients with postmenopausal osteoporosis.

METHODS: Postmenopausal patients with osteoarthritis of the hip scheduled for total hip arthroplasty, aged 50-70 years, were enrolled, including 10 postmenopausal osteoporosis patients (experimental group) and 10 patients with no postmenopausal osteoporosis (control group). During the arthroplasty, the cancellous bone specimens from the femoral head were collected. Then, immunohistochemistry and western blot assay were used to detect expression of histone demethylase (JMJD2A, JMJD2B), histone methylation (H3K9me3, H3K36me3) and ERRα.

RESULTS AND CONCLUSION: In the experimental group, the expressions of JMJD2A, JMJD2B and ERRα were from weakly positive to positive; these expressions were significantly lower in the experimental group than the control group (P < 0.05). The expressions of H3K9me3 and H3K36me3 were significantly higher in the experimental group than the control group (P < 0.05). These findings indicate that the expression of JMJD2A and JMJD2B is consistent with the expression of ERRα in the patients with postmenopausal osteoporosis, and JMJD is likely to serve as an antagonistic enzyme of osteoporosis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Tang Hong-yu, Dong Lu-jue, Huo Shao-chuan, Guo Cheng, Zhou Chi, Chen Jian-fa, Liu Yong, Wang Hai-bin. Expression of jumonji domain-containing histone demethylase 2 and estrogen-related receptor alpha in postmenopausal osteoporosis[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(2): 167-172.

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Expression of jumonji domain-containing histone demethylase 2 and estrogen-related receptor alpha in postmenopausal osteoporosis

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