Skin composite construction using adipose tissue-derived stem cells for wound healing

doi:10.3969/j.issn.2095-4344.2016.01.002

Abstract

Abstract:

BACKGROUND: There is no clear understanding on the effects of subcutaneous fat and stem cells on wound healing.
OBJECTIVE: To explore the therapeutic effects of skin composite prepared with adipose tissue-derived stem cells on skin defects.
METHODS: Epidermal cells, fibroblasts, adipose tissue-derived stem cells as seed cells and bovine collagen gel as a scaffold were used to build a complex with a variety of cells. A 6-mm diameter circular skin defect was made on the both sides of the rat back. The right side as experimental side was implanted with an 8-mm diameter multilayer skin composite, and the left side (control side) was only treated with a simple dressing.
RESULTS AND CONCLUSION: For the constructed multi-layer skin composite, the epidermal layer was continuously merged into the multi-layer, the fibroblasts evenly distributed in the corium layer, and lipid droplets existed in the fat layer in which the cells distributed uniformly. Cell aggregation was obviously observed at the junction of different layers. In the experimental side, the rate of wound healing, granulation tissue thickness, the thickness of dermis and the capillary density were significantly higher than those in the control side. Taken together, we can construct multilayer skin composites with a variety of cells as seed cells, such as epidermal cells, fibroblasts and adipose tissue-derived stem cells, and bovine collagen gel as a scaffold, which promote wound healing and increase the thickness of dermis.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Peng Xi-liang, Zhang Yu-hong, Ni Wen-qiong . Skin composite construction using adipose tissue-derived stem cells for wound healing[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2016.01.002.

Figures/Tables 4

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