Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (50): 9316-9320.doi: 10.3969/j.issn.2095-4344.2012.50.002

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Effect of mangiferin on the proliferation of rat osteoblasts

Pan Sheng-cai1, Li Xiao-feng2, Tang Yu-jin1, Xie Ke-gong1, Lan Chang-gong1, Lu Xian-zhe1   

  1. 1Department of Spinal and Bone Disease, the Affiliated Hospital, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China; 2Department of Traumatic Orthopedics and Hand Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Online:2012-12-09 Published:2013-02-16
  • Contact: Tang Yu-jin, Master, Chief physician, Department of Spinal and Bone Disease, the Affiliated Hospital, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China tangyujin196709@163.com

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Abstract:

BACKGROUND: Mangiferin and its extracts have many physiological and pharmacological effects, but whether they can promote the proliferation of osteoblasts has not been reported.
OBJECTIVE: To observe the effects of mangiferin on the proliferation of osteoblasts cultured in vitro from SD rats.
METHODS: The osteoblasts from the skull of neonatal SD rats were cultured by the second enzyme digestion. The MTT method and flow cytometry were used to observe the cytotoxicity proliferation of osteoblasts cultured in vitro by different concentrations of mangiferin at different time points.
RESULTS AND CONCLUSION: In a certain range of concentrations, different concentrations of mangiferin could promote the proliferation and differentiation of osteoblasts in a certain dose and time dependent manner. The optimal concentration of mangiferin was 40 μmol/L to promote the proliferation of osteoblasts. When the drug action time was 48 and 72 hours, 20, 40 and 80 μmol/L mangiferin could significantly promote osteoblast proliferation (P <0.01 or P < 0.05). The S-phase fraction of osteoblasts treated with 20, 40, and 80 μmol/L mangiferin was significantly increased than that of normal cells, especially those treated with 40 μmol/L mangiferin (P < 0.05). These findings indicate that in a certain range of concentrations, mangiferin can promote the proliferation of osteoblasts in vitro.