Abstract:

BACKGROUND: The molecular mechanism of deep vein thrombosis and further regression is extremely complex. Researches show that hypoxia and injury factors are involved in deep vein thrombosis and regression.
OBJECTIVE: To investigate changes in hypoxia inducible factor (HIF)-1 expression during deep vein thrombosis and regression in a rabbit model of traumatic deep vein thrombosis (TDVT).
METHODS: The bilateral femoral vein of rabbits was clamped and both lower extremities were fixed with gypsum to establish model of TDVT. SYBR®Green I fluorescence real-time quantitative PCR and ELISA dynamic detection were used to determine the change of HIF-1 mRNA and protein expression in femoral vein in the rabbits with TDVT.
RESULTS AND CONCLUSION: At 24 hours post-injury, B-ultrasonography showed that thrombosis rate was 58% and thromboembolus gradually reduced and be organized with time; from the 5th day intermittent flow signals were monitored in lumen of blood vessel with thrombosis of model group. HIF-1 expression in vein of model group gradually increased (P < 0.05 or P < 0.01), and reduced between 7-14 days (P < 0.05 or P < 0.01). Those findings indicate that HIF-1 expression is upregulated during TDVT, but downregulated during thrombolysis and organization.