Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (42): 7857-7860.doi: 10.3969/j.issn.2095-4344.2012.42.013

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Separation and identification of human umbilical vein endothelial cells and expression of their membrane molecules

Yang Jing1, Li Qian-ru1, Huang Yu-min1, Xu Hong2, Zhao Jiu-zhou3, Du Ying1   

  1. 1Department of Microbiology and Immunology, 3Department of Pathogen Biology, Basic Medical School, Zhengzhou University, Zhengzhou 450001, Henan Province, China; 2Cancer Institute, Henan Tumor Research Hospital, Zhengzhou 450003, Henan Province, China
  • Received:2012-01-11 Revised:2012-03-29 Online:2012-10-14 Published:2012-10-14
  • Contact: Du Ying, Doctor, Professor, Department of Microbiology and Immunology, Basic Medical School, Zhengzhou University, Zhengzhou 450001, Henan Province, China duying@zzu.edu.cn
  • About author:Yang Jing★, Studying for master’s degree, Department of Microbiology and Immunology, Basic Medical School, Zhengzhou University, Zhengzhou 450001, Henan Province, China yangjing2009@qq.com

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Abstract:

BACKGROUND: The tumor microenvironment affects expression of vascular endothelial cells. Critical molecular, which plays a part in tumor growth, metastasis and immunologic escape; however, its specific mechanisms remain largely unknown.
OBJECTIVE: To study the expression changes in membrane molecules of vascular endothelial cells under the influence of the tumor microenvironment.
METHODS: Human umbilical vein endothelial cells were stimulated with cultured supernatants of tumor cells at the indicated concentration for the same time and with the same concentration for the different time in vitro. Factor VIII related antigen was detected by immunocytochemistry methods. The expression of membrane molecular mRNA was detected by real-time quantitative PCR.
RESULTS AND CONCLUSION: The supernatant of hepatocellular cancer cell line smmc7721 suppressed the ability of antigen-presenting and adhesion of vascular endothelial cells. This effect was strengthened when time was increased. However, the supernatant of gastric cancer cell line SGC7901 did not have this effect; it influenced the expression of CD31, CD62E and vascular cell adhesion molecule 1. Tumor microenvironment affects the function of vascular endothelial cells by changing the expression of molecules related antigen-presenting and adhesive capacity.

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