Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (37): 6847-6850.doi: 10.3969/j.issn.2095-4344.2012.37.002

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Dynamic expression of osteoprotegerin gene in an osteoporotic rat’s segmental bone defect model

Xu Xiao-feng, Chen Hai-ning, Chen Chen, Zhao Yi-wen, Liu Zi-wen   

  1. Department of Orthopedics, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
  • Received:2012-01-24 Revised:2012-02-19 Online:2012-09-09 Published:2012-09-09
  • About author:Xu Xiao-feng, Chief physician, Associate professor, Master’s supervisor, Department of Orthopedics, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China andy.china@ foxmail.com

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Abstract:

BACKGROUND: There are many domestic reports regarding the healing principle and treatment choice of bone defects under normal conditions, but few in the case of osteoporosis.
OBJECTIVE: To observe the dynamic expression of osteoprotegerin (OPG) gene during restoration of segmental bone defects in osteoporotic rats.
METHODS: After osteoporotic models were established in all rats, the models of segmental bone defects could be established at 3 day, and 1, 2, 4, 8 weeks before we extracted the specimens. Then, the expression of OPG and alkaline phosphatase were detected by reverse transcription-PCR and immunohistochemical technique.
RESULTS AND CONCLUSION: In different stages of osteoporotic segmental bone defects, the expressions of OPG and alkaline phosphatase were increased in early and middle stages and then decreased. It is indicated that the key factors to repair osteoporotic rat's segmental bone defects is the expression of OPG.

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