Abstract:

BACKGROUND: Human periodontal ligament cells are one of the ideal seed cells in periodontal tissue engineering.
OBJECTIVE: To investigate the effect of basic fibroblast growth factor gene transfection with recombinant adeno-associated virus as a vector on the proliferation and cell cycle of cultured human periodontal ligament cells in vitro.
METHODS: Human periodontal ligament cells cultured in vitro were transfected with basic fibroblast growth factor gene mediated by recombinant adeno-associated virus, and then the transfected cells were divided into three groups: control group, recombinant adeno-associated virus group and basic fibroblast growth factor gene transfection group. Reverse transcription-PCR and western blot were used to assess the gene and protein expression of basic fibroblast growth factor before and after transfection. Cellular proliferation was determined by the cell growth curve and MTT assay. The cell cycle was analyzed by flow cytometry.
RESULTS AND CONCLUSION: There was no basic fibroblast growth factor expression in the control and recombinant adeno-associated virus groups. After transfection, the expression of basic fibroblast growth factor mRNA and protein was found in the basic fibroblast growth factor gene transfection group, the cell growth was accelerated, the cell cycle of G₀/G₁ phase was decreased and cell number in S phase was increased. There was a significant difference among the groups (P < 0.05).