Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (7): 1327-1330.doi: 10.3969/j.issn.1673-8225.2011.07.042

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Expressions of anticoagulant genes KLF2 and KLF4 in blood for prediagnosing deep venous thrombosis in rats

Yao Li-qing1, Dai Yao-jun1, Zhao Xue-ling1, Zhang Yu-bing1, Ning Ya1, Li Hong-kun1, Li Wen2   

  1. 1Department of Orthopaedics, First Affiliated Hospital of Kunming Medical University, Kunming   650032, Yunnan Province, China
    2Department of Cardiology, Yunnan First People’s Hospital, Kunming  650032, Yunnan Province, China
  • Received:2010-09-06 Revised:2010-11-12 Online:2011-02-12 Published:2011-02-12
  • Contact: Li Wen, Asscicote Chief physician, Department of Cardiology, Yunnan First People’s Hospital, Kunming 650032, Yunnan Province, China liwenkmty@yahoo. com.cn
  • About author:Yao Li-qing☆, Studying for doctorate, Associate chief physician, Department of Orthopaedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China yaoliqing98731@ yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30960389*

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Abstract:

BACKGROUND: There is lack of an effective measuring means to diagnose deep venous thrombosis (DVT) in clinic. KLF2 and KLF4 are down-expressed at prethrombotic state, which may be served as predictive molecular markers to diagnose DVT.
OBJECTIVE: To explore the feasibility of KLF2 and KLF4 as molecular markers to prediagnose DVT in rats.
METHODS: Totally 90 rats were obtained from 100 rats to establish traumatic DVT models and divided into the prethrombotic, thrombosis crest-time and non-thrombosis groups. The remained 10 rats served as control group. Rat blood was collected at each time point, and the expressions of KLF2 and KLF4 were detected by real-time PCR.
RESULTS AND CONCLUSION: The KLF2 and KLF4 mRNA expressions in the prethrombotic group and thrombosis crest-time group were lower than that of the control group. However, the KLF2 and KLF4 mRNA expressions in the non-thrombosis group was higher than that of the control group. Therefore, KLF2 and KLF4 may be candidate molecular markers for prediagnosis of DVT in rats.

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