Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (7): 1210-1214.doi: 10.3969/j.issn.1673-8225.2011.07.015

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Effect of natural and recombinant hirudin on vascular endothelial cell growth factor in random skin flap congestion models

Guo Ying-xin, Yin Guo-qian, Li Jia-quan, Xiao Han   

  1. Department of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2010-07-28 Revised:2010-08-27 Online:2011-02-12 Published:2011-02-12
  • Contact: Yin Guo-qian, Chief physician, Doctoral supervisor, Department of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China yingq61@163.com
  • About author:Guo Ying-xin☆, Studying for doctorate, Attending physician, Department of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China nngyx_2008@126. com
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2010GXNSFA013141*

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Abstract:

BACKGROUND: Flap venous congestion, even necrosis, occurs after random skin flap transplantation. Clinical and animal experiments have proved that hirudin can improve the survival rate of congestion flap.
OBJECTIVE: To investigate the protective effect of hirudin on random skin flap congestion.
METHODS: Thirty Wistar rats were established animal model of venous congestion. Dorsal random skin flap was prepared on each animal with 10 cm × 3 cm in size. The models were randomly divided into the natural hirudin, recombinant hirudin and control groups, which received subcutaneous injection of 5 U natural hirudin, recombinant hirudin or physiological saline, respectively. Vascular endothelial cell growth factor (VEGF) levels and VEGF mRNA expression were measured after surgery. The flap survival rate was calculated.
RESULTS AND CONCLUSION: Compared with the control group, the flap survival rate of the natural hirudin or recombinant hirudin group was higher (P < 0.05). The VEGF expression of the natural hirudin or recombinant hirudin group was greater than that of the control group (P < 0.05), especially greatest in the natural hirudin group. The results demonstrated that, both natural and recombinant hirudin can promote VEGF expression in random flaps, accelerate angiogenesis and increase survival rate of random flaps. The natural hirudin shows superior effects to recombinant hirudin.

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