Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (7): 1141-1145.doi: 10.3969/j.issn.1673-8225.2011.07.001

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Effect of calcitonin on femoral fracture healing in ovariectomized rats

Song Ya-qi1, 2, Zhang Liu1, Luo Yang1, Tian Fa-ming1, Cheng Tan1   

  1. 1Department of Orthopaedics, Affiliated Hospital of North China Coal Medical College, Tangshan   063000, Hebei Province, China
    2Tangshan People’s Hospital, Tangshan   063000, Hebei Province, China
  • Received:2010-08-24 Revised:2010-09-27 Online:2011-02-12 Published:2011-02-12
  • Contact: Zhang Liu, Doctor, Chief physician, Professor, Doctoral supervisor, Department of Orthopaedics, Affiliated Hospital of North China Coal Medical College, Tangshan 063000, Hebei Province, China zhliu130@sohu.com
  • About author:Song Ya-qi★, Master, Attending Physician, Department of Orthopaedics, Affiliated Hospital of North China Coal Medical College, Tangshan 063000, Hebei Province, China; Tangshan People’s Hospital, Tangshan 063000, Hebei Province, China Songyaqi07@126. com

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Abstract:

BACKGROUND: Calcitonin can activates adenylyl cyclase protein kinase A pathway and phospholipase C pathway, inhibits activity of osteoclast, may treats osteoporotic fracture.
OBJECTIVE: To observe the effect of calcitonin on femoral fracture healing in ovariectomized rats.
METHODS: Ovariectomized SD rat right femur fracture model was constructed, normal saline and calcitonin (16 IU/kg) were injected subcutaneously in the neck once per two days, respectively. Right bone mineral density (BMD) was measured at 3 weeks and 6 weeks after fracture, stained with hematoxylin- eosin (HE) and tartrate-resistant acid phosphatase (TRAP) staining, as well as bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor (VEGF) immunohistochemistry staining.
RESULTS AND CONCLUSION: Tartrate-resistant acid phosphataste positive staining cells integral absorbance values of rats received calcitonin treatment after fracture was decreased significantly compared with rats received saline treatment (P < 0.05). SD rats were randomly divided into ovariectomy + fracture + calcitonin (experimental) group and ovariectomy + fracture + saline (control) group. At 3 weeks after fracture, fracture lines were clearly in both experimental group and control group, there was no significant difference in callus volume; endochondral ossification was dominant in fracture healing, there was no significant difference in BMD (P < 0.05). At 6 weeks after fracture, fracture lines were ambiguous in both experimental group and control group; there was no difference in callus volume, bone trabeculae arranged orderly, BMD of right femur increased significantly in experimental group compared with control group (P< 0.05). There was no significant difference of BMP-2 and VEGF between experimental group and control group at 3 weeks and 6 weeks after fracture (P > 0.05). The results demonstrated that calcitonin can inhibits osteoclast cell at fracture cite in ovariectomized rats, but has no significant effect on the promotion of femoral fracture healing in ovariectomized rats.

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