Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (5): 855-860.doi: 10.3969/j.issn.1673-8225.2011.05.023

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Effects of salvia miltiorrhiza on survival rate of flaps with ischemia reperfusion injury

He Zhi, Qiu Shu-lin   

  1. Department of Burns and Plastic Surgery, People’s Hospital of Hebei Province, Shijiazhuang  050051, Hebei Province, China
  • Received:2010-07-19 Revised:2010-09-01 Online:2011-01-29 Published:2011-01-29
  • Contact: Qiu Shu-lin, Professor, Master’s supervisor, Department of Burns and Plastic Surgery, People’s Hospital of Hebei Province, Shijiazhuang 050051, Hebei Province, China qiushulin8229@sina.com.cn
  • About author:He Zhi★, Master, Department of Burns and Plastic Surgery, People’s Hospital of Hebei Province, Shijiazhuang 050051, Hebei Province, China modernhuatuo@163.com
  • Supported by:

    Research and Development of Science and Technology Plan of Hebei Province, No. 09276102D-31*

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Abstract:

BACKGROUND: Salvia miltiorrhiza (SM) is considered to be helpful to prevent flap ischemia-reperfusion injury (IR), but the specific mechanism is not clear.
OBJECTIVE: To observe the protective effects of SM injection on the flap IR injury in rats and to explore its mechanism.
METHODS: Totally 48 Wistar rats were randomly divided into 3 groups, with 16 animals in each group. No perfusion was performed in the control group. Physiological saline (2 mL/kg) was intraperitoneal injected into rats at 3 days before until 5 days after operation in the IR group. The same volume of SM injection was injected into rats in the SM-IR group. The contents of malonaldehyde, myeloperoxidase, intercellular adhesion molecule 1 (ICAM-1) and nuclear factor-κB of the island skin flap IR models were measured, and the survival rate of flap was observed using image analysis.
RESULTS AND CONCLUSION: The expressions of malonaldehyde, myeloperoxidase, ICAM-1 and nuclear factor-κB in the IR group, SM-IR group after reperfusion 2, 8, 24 hours and the control group after 10, 16, 32 hours showed: IR group > SM-IR group > control group (P < 0.01). The survival rate of flap was: IR group < SM-IR group < control group (P < 0.01). In a conclusion, salvia miltiorrhiza may inhibit NF-κB activation pathway and its own ring-inflammatory response that has the anti-oxidation, improve the body's overall antioxidant capacity, reduce flap ischemia-reperfusion injury, thus, promote flap survival.

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