Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (51): 9501-9506.doi: 10.3969/j.issn.1673-8225.2010.51.001

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Effect of salvianolic acid B-loaded hydroxyapatite sustained release spherules on the activity of osteoblasts

Lan Lin1, Zhi Wei1, Huang Jing1, Weng Jie1, Lu Xiao-ying1, Zhang Cong2   

  1. 1 Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu   610031, Sichuan Province, China; 2 The 452 Hospital of Chinese PLA, Chengdu   610031, Sichuan Province, China
  • Online:2010-12-17 Published:2010-12-17
  • Contact: Zhang Cong, Doctor, Professor, Doctoral supervisor, Chief physician, the 452 Hospital of Chinese PLA, Chengdu 610031, Sichuan Province, China Cong60@hotmail.com
  • About author:Lan Lin★, Studying for master’s degree, Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan Province, China truly100@sina.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30870630*; Army “Eleventh Five-Year” Scientific and Technological Project, No. 06G049*; Young Teacher Initiative Project for Research of Southwest Jiaotong University, No. 209Q007*

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Abstract:

BACKGROUND: Hydroxyapatite (HA) spherules, a new type of bone repair material, have been prepared in preliminary experiment. Bio-ceramic scaffold in a super-critical size can be constructed by adjusting HA spherules size and micro-structure. Loading some drugs that can promote bone healing on the scaffold, can control the local concentration of drugs and then improve efficiency, increase specificity, reduce side effects, and accelerate bone healing.
OBJECTIVE: To investigate the influence of salvianolic acid B (Sal B)-loaded HA sustained-release spherules on the activity of osteoblasts cultured in vitro.
METHODS: Osteoblasts were isolated from SD rats and co-cultured with HA spherules, Sal B-loaded HA sustained release spherules, chitosan-coated Sal B-loaded HA sustained release spherules respectively. The proliferation of rat osteoblasts was evaluated with Alamar Blue assay; The alkaline phosphatase activities were measured to detect the differentiation of osteoblasts. Expression of collagen Ⅰ and osteocalcin were examined with immunocytochemistry.
RESULTS AND CONCLUSION: Sal B-loaded HA sustained release spherules coated with chitosan could enhance significantly the proliferation of rat osteoblasts and alkaline phosphatase expression, the effect duration was longer than that of HA group and Sal B-loaded HA spherules without chitosan. There was no significant difference in collagen Ⅰ and osteocalcin compared with HA group. Sal B-loaded HA spherules without chitosan made cells shrinkage and death because of the toxicity to the cells induced by drug sudden release from the second day. Sal B-loaded HA sustained release spherules coated with chitosan can enhance the proliferation and differentiation of rat osteoblasts for a long term, have no influence on the bioactivity of osteoblasts and also can maintain the bioactivity of osteoblasts.

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