Abstract:

BACKGROUND: As a new dermal substitute, acellular dermal matrix (ADM) has been extensively used in repair of tissue defects. But there are no reports about the expression of basic fibroblast growth factor (bFGF) during long-dated biological transition of ADM.
OBJECTIVE: To discuss the rule of the bFGF expression in long-dated biological transition of collagen lattice in vivo.
METHODS: A total of 21 Wistar rats were applied to prepare ADM. A wound at 2.5 cm×2.5 cm was made to deep fascia surface on left side of spine cord in 84 rats. ADM was transplanted to the back of Wistar rats, while contralateral normal skin served as the control. Samples were acquired at 3, 5, 7, 10 days, 2, 3, 4, 6, 8, 10 weeks, 4, 6, 8, 10 months after operation. The bFGF expression was detected using immunohistochemical method.
RESULTS AND CONCLUSION: Expression of bFGF came to appear in 3 days, and reached the peak in 10 days, then became stable in 4 weeks, after 8 weeks the expression of bFGF was the same with normal skin. In the course of long-dated biological transition of ADM, its rebuilding begins from the border of ADM gradually to the center; the rebuilding will become stable at 6-8 weeks after transplantation, then the ADM is considered to be autologous tissue and participates in the metabolic process of normal tissue.