Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (47): 8818-8823.doi: 10.3969/j.issn.1673-8225.2010.47.019

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In vitro experiment of modified nano-bone adhesive

Zhao Zhe 1,2, Lei Ming2, Xiao De-ming2, Xiong Jian-yi2, Ma Jing-ye3, Xu Yun3, Chen Xu-hong3, Peng Fu-ning3   

  1. 1 Guangzhou Medical College, Guangzhou  510182, Guangdong Province, China; 2 Department of Orthopaedics, 3 Central Laboratory, The Second People’s Hospital of Shenzhen, Shenzhen   518035, Guangdong Province, China
  • Online:2010-11-19 Published:2010-11-19
  • Contact: Xiao De-ming, Doctoral supervisor, Chief physician, Department of Orthopaedics, The Second People’s Hospital of Shenzhen, Shenzhen 518035, Guangdong Province, China Xiaodm_1@163.com
  • About author:Zhao Zhe★, Studying for master’s degree, Guangzhou Medical College, Guangzhou 510182, Guangdong Province, China; Department of Orthopaedics, The Second People’s Hospital of Shenzhen, Shenzhen 518035, Guangdong Province, China Zhe218@yahoo.com.cn
  • Supported by:

     a grant by Science and Technology Bureau of Guangdong Province, No. 2008B030301284*

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Abstract:

BACKGROUND: Currently α-cyanoacrylate glue can be used in many clinical fields, but the application is limited in orthopedics due to some shortcomings of the glue itself.
OBJECTIVE: To develop a nano-bone adhesive by modifying common medical glue, to evaluate its cytotoxicity and mechanical property. 
METHODS: Rabbit bone marrow stromal stem cells were directly implanted with nano-bone adhesive and common medical glue, the growth of these cells on the surface of the two glues was observed by inverted microscope and scanning electron microscope. The extracting solution of the two glues was collected to culture cells. At 0, 6, 12, 18, 24 hours (short-term assay, viability) and 1-7 days (long-term assay, survival), the cells were examined everyday. Standard polyvinyl chloride tubes were cohered by the two glues respectively, to compare their adhesive strength. Their consolidation time on different contract surfaces was also accounted. 
RESULTS AND CONCLUSION: After three times of culture medium replacement, the cells can be grow on the surface of nano-bone adhesive and medical glue. Both had some cytotoxicity that was not significant difference. The mechanical strength of nano-bone adhesive was stronger than the medical glue, and the consolidation time of the former was also faster than the latter. The cytotoxicity of modified nano-bone adhesive is not higher than the medical glue, but the mechanical strength and consolidation are more superior. The former also can stimulate the ossification.

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