Abstract:

BACKGROUND: Based on the theory of biomineralized organic matrix template controls inorganic crystal growth, human hard tissue repair materials constructed using molecular bionic technology is one of the research hotspots of biological materials. But there is no report regarding polyelectrolyte complex organic matrix plate constructed for biomimetic synthesis of bone tissue repair materials.
OBJECTIVE: Polyelectrolyte complex hydrogel formed by reacting polycation chitosan and polyanion derivate phosphorylated chitosan is used as the pre-tissue biomineralized organic matrix template to biomimetic synthesis of the biomaterials.
METHODS: Firstly natural polycation polysaccharide chitosan was modified through phosphorylation, to synthesize polyanion derivate phosphorylated chitosan; then polyelectrolyte complex hydrogel was formed from equal volumes of phosphorylated chitosan solution and polycation chitosan solution. The obtained polyelectrolyte complex hydrogel was soaked in supersaturated CA/P solution to induce hydroxyapatite crystals deposit and grow under the regulation of hydrogel matri. It was characterized with fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscope.
RESULTS AND CONCLUSION: The porous polyelectrolyte complex hydrogel formed by the interaction between polycation chitosan and polyanion phosphorylated chitosan can induce hydroxyapatite crystals nucleated and grew on the fiber surfaces of the hydrogel, thus biomimetic synthesizing a new composite biomaterial for bone repairing.