Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (47): 8751-8754.doi: 10.3969/j.issn.1673-8225.2010.47.003

Previous Articles     Next Articles

Survival and differentiation of neural stem cells adhered to muscle basal lamina tissue engineering scaffolds

Song Yu 1,2 , Liu Jia-mei2, Xue Hui2, Zhang Xiu-ying3, Tian Xiao-wei2   

  1. 1 Department of Anatomy, Changchun Medical College, Changchun   130031, Jilin Province, China; 2 Department of Histology & Embryology, Norman Bethune College of Medicine, Jilin University, Changchun   130021, Jilin Province, China; 3 Department of Fundamental Nursing, School of Nursing, Jilin University, Changchun   130021, Jilin Province, China.
  • Online:2010-11-19 Published:2010-11-19
  • Contact: Liu Jia-mei, Professor, Associate professor, Master’s supervisor, Department of Histology & Embryology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, Jilin Province, China Liujiamei100@yahoo.com.cn
  • About author:Song Yu★, Studying for master’s degree, Lecturer, Department of Anatomy, Changchun Medical College, Changchun 130031, Jilin Province, China; Department of Histology & Embryology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, Jilin Province, China songyumc@163.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30970739*; International Science and Technology Cooperation Program of Science and Technology Bureau of Jilin Province, No.20090726*

PDF

611

Abstract:

BACKGROUND: Many researches have shown that neural stem cell can promote the rat neural function recover of spinal injury. Muscle basal lamina has a good biocompatibility and degradability. Therefore the combination of muscle and neural stem cells to be a new nerve tissue engineering scaffold has been considered. 
OBJECTIVE: To observe the survival and differentiation of neural stem cells as seed cells adhered to the muscle basal lamina as a scaffold.
METHODS: Rat neural stem cells were isolated, cultured and then identified in vitro. Following preparation of acellular bone muscle basal lamina scaffolds by chemical extraction method, the neural stem cells were implanted into the scaffolds. After seven days of cultivation, the survival and differentiation of neural stem cells were detected by immunohistochemical technique, and the ultra-structure was observed under a scanning electron microscope.
RESULTS AND CONCLUTION: On the fifth day of cultivation, a great amount of neurospheres were seen by Nestin immunofluorescence. Following 7-day culture and differentiation in serum medium, the neural stem cells were used for anti-NF and anti-GFAP immunofluorescent staining. NF and GFAP positive cells were found under the microscope, which indicated that the cultured neural stem cells owned multi-lineage differentiation potential. Hemotoxylin and eosin (HE) staining showed that myocytes disappeared from the muscle basal lamina and there were roughly parallel tunnels inside the muscle basal lamina scaffolds. Immunofluorescent staining showed that the neural stem cells adhered to the scaffolds still had the characteristics of stem cells and could differentiate into neurons and neurogliocytes. The neural stem cells were firmly attached to the muscle basal lamina under the scanning electron microscope. These findings indicated that nerve tissue engineering scaffold has a good biocompatibility and can be used for in vivo transplantation to treat nervous system diseases, such as spinal cord injury.

CLC Number: