Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (47): 8747-8750.doi: 10.3969/j.issn.1673-8225.2010.47.002

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Effects of fibrin glue on the proliferation and viability of adipose tissue-derived stem cells

Zhang Xue-lian1, Ma Yi-tong1, Wang Chang-yong2, Ma Xiang1, Adila•Azhati1, Liu Fen1, Chen Bang-dang1   

  1. 1 Department of Coronary Artery Disease, Heart Center of the First Affiliated Hospital, Xinjiang Medical University, Urumqi   830054, Xinjiang Uygur Autonomous Region, China; 2 Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing   100850, China
  • Online:2010-11-19 Published:2010-11-19
  • Contact: Ma Yi-tong, Doctor, Chief physician, Professor, Department of Coronary Artery Disease, Heart Center of the First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China myt-xj@163.com
  • About author:Zhang Xue-lian☆, Studying for doctorate, Associate chief physician, Department of Coronary Artery Disease, Heart Center of the First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China zxuelian2003@sina.com
  • Supported by:

    Science and Technology Support Plan for Xinjiang Uygur Autonomous Region, No. 200891127*

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Abstract:

BACKGROUND: Many biological scaffolds are used in carrying seed cells, however, there is no criterion about the co-culture of fibrin glue with adipose tissue-derived stem cells and the evaluation of the proliferation and viability of seed cells on the scaffolds.
OBJECTIVE: To investigate the effects of fibrin glue on the proliferation and viability of rat adipose tissue-derived stem cells cultured in vitro, and to provide the experimental evidence for injectable tissue engineered myocardial treatment for myocardial infarction.
METHODS: Adipose tissue-derived stem cells were isolated from rats and then cultured. Their immunophenotype was identified by flow cytometry. Adipose tissue-derived stem cells were co-cultured with fibrin glue. The proliferation of adipose tissue-derived stem cells were tested by MTT assay and cell viability was assessed with LIVE/DEAD fluorescence double stain.
RESULTS AND CONCLUSION: The adipose tissue-derived stem cells of rats were positive for CD90 and CD105, but negative for CD34 and CD45. MTT assay showed that adipose tissue-derived stem cells proliferated faster in fibrin glue group than the control group (P < 0.05); LIVE/DEAD fluorescence double stain showed that adipose tissue-derived stem cells grew well in fibrin glue. The viability of adipose tissue-derived stem cells maintained > 90% and the mortality was < 7% at 24 hours and 72 hours. Adipose tissue-derived stem cells well proliferate and survive in fibrin glue, fibrin scaffold may work as a carrier in deliverying adipose tissue-derived stem cells for injectable myocardial tissue engineering study.

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