Abstract:

BACKGROUND: The over expression of synoviolin gene leads to synoviocyte overgrowth and proliferation. Thus, it is possible to realize synovialization of non-synovial tendon by importing synoviolin gene into synoviocytes, which can prevent tendon adhesion.
OBJECTIVE: To explore the effects of synoviolin gene modified synoviocyte on the prevention of tendon adhesion.
METHODS: Totally 30 Leghom chickens were prepared for tendon injury models and randomly divided into the experimental and control groups. The adenovirus vector containing synoviolin gene was injected into the remnant of tendon sheathes. Gross observation, histological examination and biomechanical test were performed at 1, 3 and 8 weeks after operation.
RESULTS AND CONCLUSION: The adhesion at the fourth toe, gliding distance of the third toe and stimulate active flexion of the experimental group were superior to those in the control group (P < 0.05). Compared with the control group, the typeⅠcollagen content was decreased in the experimental group, and the ratio of type Ⅲ collagen was declined. The findings demonstrated that synoviolin gene modified synoviocyte could transform the nonsynovial membrane tendon into a synovial membrane tendon, suggesting that it is an effective method for preventing flexor tendon from adhesion after operation.