Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (42): 7807-7810.doi: 10.3969/j.issn.1673-8225.2010.42.005

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Bone marrow mesenchymal stem cells transfected by human bone morphogenetic protein-2 and bioactive glass ceramics composite material for repair of skull defects

Zheng De-yu1, Yang Yi-yong1, Zhao Kai1, Zheng Shi-jie1, Zhang Yu-hua2, Qin Shu-jian1   

  1. 1 Department of Anatomy, Liaoning Medical University, Jinzhou   121001, Liaoning Province, China; 2 Department of Bone Surgery, Central Hospital of Tiedong District of Anshan, Anshan   114003, Liaoning Province, China
  • Online:2010-10-15 Published:2010-10-15
  • About author:Zheng De-yu☆, Doctor, Associate professor, Department of Anatomy, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China zheng_deyu2000@yahoo.com.cn
  • Supported by:

    Youth Fund by Liaoning Provincial Education Bureau, No. 05L140*; Innovation Team Program by Liaoning Provincial Education Bureau, No. 2010T061*

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Abstract:

BACKGROUND: The current studies have reported in vitro cultured bone marrow mesenchymal cells combined with scaffolds to repair the bone defects. However, the bone formation in situ remains un-satisfactory due to lack of bone growth factor in such composite.
OBJECTIVE: To investigate the in situ osteogenic effect of artificial bony graft, formed by bone marrow mesenchymal stem cells (BMSCs) modified by human bone morphogenetic protein-2 (hBMP-2) and bioactive glass ceramics (BGC) for repairing skull defects of rabbits.
METHODS: The BMSCs transfected hBMP-2 gene were cryoopreserved and resuscitated, then composited with BGC to prepare artificial bony graft. Rabbits skull defect models, created on the both parietal bones, were filled with the artificial bony graft, as the experimental group. While those treated by blank plasmid-transfected BMSCs + BGC and simple BMSCs + BGC, as the control groups. Blank control group was also set. The bone defects were observed by gross observation, X-ray, immunohistochemical and biochemical detections in the 4th, 8th, 12th weeks after operation respectively.
RESULTS AND CONCLUSION: At four weeks after BMSCs transfected with hBMP-2 and BGC composite materials were transplanted into the skull defect, the interspace of artificial bone and bone defect were mainly filled by high density shadow in the experiment group, at 12 weeks, the space were completely filled by high density shadow. The great part of bony trabeculae were connected each other at 8 weeks, and bony trabeculae become thicker and formed as a network and new marrow regenerated at 12 weeks. The outcomes of biochemistry were coincided with the outcome of immunohistochemistry. The bone formation and osteogenesis activity were markedly better than those in control groups. The artificial bony graft formed by BMSCs transfected by hBMP-2 and BGC may satisfy the requirement of bone tissue engineering and has good effects for accelerating the bone defects healing.

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